Ludmir J, Alvarez J G, Mennuti M T, Gabbe S G, Touchstone J C
Am J Obstet Gynecol. 1987 Jul;157(1):84-8. doi: 10.1016/s0002-9378(87)80351-4.
Cholesteryl palmitate was measured by thin-layer chromatography in 98 amniotic fluid samples obtained from pregnancies ranging from 15 to 40 weeks. Pregnancies complicated by diabetes mellitus or Rh sensitization were excluded. Cholesteryl palmitate concentration increased with gestational age from values less than 5 micrograms/ml at 15 weeks to maximum values of 384 micrograms/ml at term. Seventy-three patients were delivered of their infants within 24 hours of sampling. The mean concentration of cholesteryl palmitate for 55 infants without respiratory distress syndrome was 123.70 +/- 12.21 micrograms/ml (SD). For neonates that developed respiratory distress syndrome (n = 18), the mean concentration was 22.66 +/- 6.16 micrograms/ml (SD). This difference is significant (p less than 0.001). For all patients delivered, respiratory distress syndrome was not seen when the cholesteryl palmitate concentration was greater than 41 micrograms/ml (mean + 3 SD). All infants with a concentration below 38 micrograms/ml developed respiratory distress syndrome. We conclude that measurement of cholesteryl palmitate concentration in amniotic fluid might be another reliable method of assessing fetal lung maturity and may often improve specificity when compared with other tests of fetal lung maturity.
采用薄层色谱法对98份孕15至40周羊水样本中的胆固醇棕榈酸酯进行了测定。排除患有糖尿病或Rh致敏的妊娠病例。胆固醇棕榈酸酯浓度随孕周增加，从孕15周时低于5微克/毫升升至足月时最高384微克/毫升。73名患者在采样后24小时内分娩。55名无呼吸窘迫综合征婴儿的胆固醇棕榈酸酯平均浓度为123.70±12.21微克/毫升（标准差）。对于发生呼吸窘迫综合征的新生儿（n = 18），平均浓度为22.66±6.16微克/毫升（标准差）。这种差异具有显著性（p<0.001）。对于所有分娩的患者，当胆固醇棕榈酸酯浓度大于41微克/毫升（均值 + 3标准差）时未观察到呼吸窘迫综合征。所有浓度低于38微克/毫升的婴儿均发生了呼吸窘迫综合征。我们得出结论，测定羊水胆固醇棕榈酸酯浓度可能是评估胎儿肺成熟度的另一种可靠方法，与其他胎儿肺成熟度检测方法相比，其特异性可能常常更高。
