The University of Tokyo, Tokyo, Japan.
Japan Small Animal Medical Center, Saitama, Japan.
Vet Pathol. 2022 Nov;59(6):931-939. doi: 10.1177/03009858221120010. Epub 2022 Sep 2.
The expression of cytotoxic molecules in feline intestinal T-cell lymphoma cells was examined immunohistochemically using endoscopic samples of 50 cases. Cases included 14 large-cell lymphomas (LCLs) and 36 small-cell lymphomas (SCLs). Most LCL and some SCL exhibited marked erosion and villous atrophy. Clonal T-cell receptor (TCR) gene rearrangement was detected in 10/14 (71%) LCL cases and 33/36 (92%) SCL cases. No clonal immunoglobulin heavy chain (IgH) gene rearrangement was detected. Immunohistochemically, all cases were positive for CD3 and negative for CD79α, CD30, CD56, and Foxp3. LCLs were positive for CD8 in 13/14 cases (93%), T-cell intracellular antigen 1 (TIA1) in 14/14 cases (100%), and granzyme B in 6/14 cases (43%). SCLs were positive for CD8 in 28/36 cases (78%), TIA1 in 33/36 cases (92%), and granzyme B in 2/36 cases (6%). TIA1- and granzyme B-positive neoplastic lymphocytes were predominantly observed in the mucosal epithelium of 10/50 cases (20%) and 6/50 cases (12%), respectively. No significant differences in survival time were found based on cell size or epitheliotropism. However, cases with TIA1 and/or granzyme B neoplastic lymphocytes predominantly in the mucosal epithelium had significantly shorter survival times ( < .05), suggesting that mucosal epithelium infiltration of neoplastic cells with a cytotoxic immunophenotype is a negative prognostic factor. Therefore, intraepithelial cytotoxic lymphocytes may be associated with mucosal injury and impaired intestinal function, leading to a poor prognosis in cats with intestinal T-cell lymphoma.
本研究通过对 50 例猫肠道 T 细胞淋巴瘤病例的内镜样本进行免疫组织化学检查，研究细胞毒性分子的表达情况。这些病例包括 14 例大细胞淋巴瘤（LCL）和 36 例小细胞淋巴瘤（SCL）。大多数 LCL 和一些 SCL 表现出明显的糜烂和绒毛萎缩。在 14 例 LCL 病例和 33 例 SCL 病例中检测到克隆 T 细胞受体（TCR）基因重排。未检测到克隆免疫球蛋白重链（IgH）基因重排。免疫组织化学检查结果显示，所有病例均 CD3 阳性，CD79α、CD30、CD56 和 Foxp3 阴性。14 例 LCL 病例中有 13 例（93%）CD8 阳性，14 例（100%）TIA1 阳性，6 例（43%）颗粒酶 B 阳性。36 例 SCL 病例中有 28 例（78%）CD8 阳性，33 例（92%）TIA1 阳性，2 例（6%）颗粒酶 B 阳性。在 10 例（20%）和 6 例（12%）病例中，分别有 10/50 例和 6/50 例黏膜上皮中 TIA1 和/或颗粒酶 B 阳性肿瘤淋巴细胞为主。根据细胞大小或上皮亲嗜性，未发现生存时间存在显著差异。然而，TIA1 和/或颗粒酶 B 阳性肿瘤淋巴细胞主要位于黏膜上皮的病例生存时间明显缩短（<.05），表明具有细胞毒性免疫表型的肿瘤细胞浸润黏膜上皮是一个不良预后因素。因此，肠道 T 细胞淋巴瘤猫的黏膜上皮内浸润的细胞毒性淋巴细胞可能与黏膜损伤和肠道功能障碍有关，导致预后不良。
