Li Baochen, Chen Qian, Xi Lei
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, China.
Guangdong Provincial Key Laboratory of Advanced Biomaterials, Southern University of Science and Technology, Shenzhen, Guangdong, China.
J Biophotonics. 2022 Dec;15(12):e202200196. doi: 10.1002/jbio.202200196. Epub 2022 Sep 14.
Blood vessels that deliver nutrients and oxygen over the entire body is essential for bone homeostasis. Especially, for the bone recovery, long-term in vivo vascular imaging is desirable. Here, we propose an optical and ultrasonic transparent bone window, which allows repeated, chronic monitoring of bone angiogenesis in mouse tibia defect. A metal ring with an outer diameter of 2 mm and an inner diameter of 1 mm is bonded with a silicone-based polydimethylsiloxane (PDMS) film and cover the bone surface, which can effectively eliminate the inflammation caused by repeated wound opening before imaging. We make a bone defect model in mouse tibia, and employ an optical resolution photoacoustic microscopy (ORPAM) to provide a high-resolution, label-free, long-term, in vivo observation of the bone vascularization during the bone defect healing. The results suggest that the artificial bone window can remain stable for inspection and play positive role for bone repair.
为全身输送营养和氧气的血管对于骨稳态至关重要。特别是对于骨修复而言，长期的体内血管成像很有必要。在此，我们提出了一种光学和超声透明骨窗，它能够对小鼠胫骨缺损处的骨血管生成进行反复、长期的监测。一个外径为2毫米、内径为1毫米的金属环与基于硅酮的聚二甲基硅氧烷（PDMS）膜粘结在一起并覆盖骨表面，这可以有效消除成像前因反复打开伤口而引起的炎症。我们在小鼠胫骨上制作骨缺损模型，并采用光学分辨率光声显微镜（ORPAM）对骨缺损愈合过程中的骨血管化进行高分辨率、无标记、长期的体内观察。结果表明，人工骨窗在检查过程中能够保持稳定，并对骨修复起到积极作用。
