Gao Jingyu, Xie Longli, Peng Yu, Li Mo, Li Jingming, Ni Yuanying, Wen Xin
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
National Engineering Research Center for Fruit and Vegetable Processing, Beijing 100083, China.
Foods. 2024 Mar 1;13(5):777. doi: 10.3390/foods13050777.
Mung beans contain abundant flavonoids like vitexin and isovitexin, which contribute to their strong bioactivities, such as antioxidant effects, so efforts should focus on extracting bioactive flavonoids as well as aligning with the goal of green extraction for specific applications. Deep eutectic solvent coupled with ultrasound-assisted extraction (DES-UAE) was applied to extract flavonoids from mung beans, and eight different DESs were compared on the extraction yield. In addition, the traditional extraction method with 30% ethanol was performed as the reference. The results showed that ethylene glycol-glycolic acid achieved the highest yield among all the DESs, 1.6 times that of the reference values. Furthermore, the DES-UAE parameters were optimized as a 60 mL/g liquid-solid ratio, 30% water content in DES, 200 W ultrasonic power, 67 °C ultrasonic temperature, and 10 min extraction time, leading to the DES extract with the maximum extraction yield of 2339.45 ± 42.98 μg/g, and the significantly stronger DPPH and ABTS radical scavenging ability than the traditional extract. Therefore, employing DES and ultrasonic extraction together offers a green method for extracting flavonoids from mung beans, advancing the development and utilization of plant-derived effective components in a sustainable manner.
绿豆含有丰富的黄酮类化合物，如牡荆素和异牡荆素，这些成分赋予了它们强大的生物活性，如抗氧化作用，因此应致力于提取生物活性黄酮类化合物，并符合特定应用的绿色提取目标。采用深共晶溶剂结合超声辅助提取法（DES-UAE）从绿豆中提取黄酮类化合物，并比较了8种不同的深共晶溶剂的提取率。此外，以30%乙醇的传统提取方法作为对照。结果表明，乙二醇-乙醇酸在所有深共晶溶剂中提取率最高，是对照值的1.6倍。此外，DES-UAE的参数优化为液固比60 mL/g、深共晶溶剂含水量30%、超声功率200 W、超声温度67℃、提取时间10 min，所得DES提取物的最大提取率为2339.45±42.98μg/g，其DPPH和ABTS自由基清除能力明显强于传统提取物。因此，将深共晶溶剂和超声提取结合使用为从绿豆中提取黄酮类化合物提供了一种绿色方法，以可持续的方式推动植物源有效成分的开发和利用。
