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比较小鼠双亲合子和单亲合子胚胎的母体蛋白谱。

Comparative maternal protein profiling of mouse biparental and uniparental embryos.

机构信息

Department of Histology and Embryology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P. R. China.

Central Laboratory, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P. R. China.

出版信息

Gigascience. 2022 Sep 3;11. doi: 10.1093/gigascience/giac084.

DOI:10.1093/gigascience/giac084
PMID:36056732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9440387/
Abstract

BACKGROUND

Maternal proteins have important roles during early embryonic development. However, our understanding of maternal proteins is still very limited. The integrated analysis of mouse uniparental (parthenogenetic) and biparental (fertilized) embryos at the protein level creates a protein expression landscape that can be used to explore preimplantation mouse development.

RESULTS

Using label-free quantitative mass spectrometry (MS) analysis, we report on the maternal proteome of mouse parthenogenetic embryos at pronucleus, 2-cell, 4-cell, 8-cell, morula, and blastocyst stages and highlight dynamic changes in protein expression. In addition, comparison of proteomic profiles of parthenogenotes and fertilized embryos highlights the different fates of maternal proteins. Enrichment analysis uncovered a set of maternal proteins that are strongly correlated with the subcortical maternal complex, and we report that in parthenogenotes, some of these maternal proteins escape the fate of protein degradation. Moreover, we identified a new maternal factor-Fbxw24, and highlight its importance in early embryonic development. We report that Fbxw24 interacts with Ddb1-Cul4b and may regulate maternal protein degradation in mouse.

CONCLUSIONS

Our study provides an invaluable resource for mechanistic analysis of maternal proteins and highlights the role of the novel maternal factor Fbw24 in regulating maternal protein degradation during preimplantation embryo development.

摘要

背景

母体蛋白在早期胚胎发育中具有重要作用。然而，我们对母体蛋白的理解仍然非常有限。在蛋白质水平上对小鼠单倍体（孤雌生殖）和二倍体（受精）胚胎进行综合分析，创建了一个蛋白质表达图谱，可用于探索小鼠植入前的发育。

结果

我们使用无标记定量质谱（MS）分析，报告了在原核、2 细胞、4 细胞、8 细胞、桑椹胚和囊胚阶段的小鼠孤雌生殖胚胎的母体蛋白质组，并强调了蛋白质表达的动态变化。此外，比较孤雌生殖胚胎和受精胚胎的蛋白质组图谱，突出了母体蛋白的不同命运。富集分析揭示了一组与皮质下母体复合物密切相关的母体蛋白，我们报告说在孤雌生殖胚胎中，其中一些母体蛋白逃脱了蛋白质降解的命运。此外，我们鉴定了一种新的母体因子-Fbxw24，并强调其在早期胚胎发育中的重要性。我们报告说，Fbxw24 与 Ddb1-Cul4b 相互作用，可能调节小鼠中的母体蛋白降解。

结论

我们的研究为母体蛋白的机制分析提供了宝贵的资源，并强调了新型母体因子 Fbw24 在调节植入前胚胎发育中母体蛋白降解中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/d024b1e78c8a/giac084fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/e534ea0c5784/giac084fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/c2452bb1eedd/giac084fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/0e989113da6c/giac084fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/6c97bb787333/giac084fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/92300a6a6264/giac084fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/006972dd8c6d/giac084fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/4058a75ffa8c/giac084fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/d024b1e78c8a/giac084fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/e534ea0c5784/giac084fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/c2452bb1eedd/giac084fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/0e989113da6c/giac084fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/6c97bb787333/giac084fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/92300a6a6264/giac084fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/006972dd8c6d/giac084fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/4058a75ffa8c/giac084fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a284/9440387/d024b1e78c8a/giac084fig8.jpg

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