Tangudu Chandra S, Hargett Alissa M, Laredo-Tiscareño S Viridiana, Smith Ryan C, Blitvich Bradley J
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.
Department of Entomology, College of Agriculture and Life Sciences, Iowa State University, Ames, IA, USA.
Arch Virol. 2022 Dec;167(12):2577-2590. doi: 10.1007/s00705-022-05586-5. Epub 2022 Sep 3.
To increase our understanding of the diversity of the mosquito virome, 6956 mosquitoes of five species (Culex erraticus, Culex pipiens, Culex restuans, Culex tarsalis, and Culex territans) collected in Iowa in the United States in 2017 and 2020 were assayed for novel viruses by performing polyethylene glycol precipitation, virus isolation in cell culture, and unbiased high-throughput sequencing. A novel virus, provisionally named "Walnut Creek virus", was isolated from Cx. tarsalis, and its genomic sequence and organization are characteristic of viruses in the genus Hapavirus (family Rhabdoviridae). Replication of Walnut Creek virus occurred in avian, mammalian, and mosquito, but not tick, cell lines. A novel virus was also isolated from Cx. restuans, and partial genome sequencing revealed that it is distantly related to an unclassified virus of the genus Phytoreovirus (family Sedoreoviridae). Two recognized viruses were also isolated: Culex Y virus (family Birnaviridae) and Houston virus (family Mesoniviridae). We also identified sequences of eight novel viruses from six families (Amalgaviridae, Birnaviridae, Partitiviridae, Sedoreoviridae, Tombusviridae, and Totiviridae), two viruses that do not belong to any established families, and many previously recognized viruses. In summary, we provide evidence of multiple novel and recognized viruses in Culex spp. mosquitoes in the United States.
为增进我们对蚊子病毒群落多样性的了解，我们对2017年和2020年在美国爱荷华州采集的5种蚊子（不定库蚊、致倦库蚊、雷氏库蚊、西方马脑炎媒介库蚊和环跗库蚊）的6956只蚊子进行了新型病毒检测，检测方法包括聚乙二醇沉淀、细胞培养中的病毒分离以及无偏差高通量测序。从西方马脑炎媒介库蚊中分离出一种新型病毒，暂命名为“核桃溪病毒”，其基因组序列和结构具有弹状病毒科正呼肠孤病毒属病毒的特征。核桃溪病毒可在禽类、哺乳动物和蚊子的细胞系中复制，但不能在蜱的细胞系中复制。还从雷氏库蚊中分离出一种新型病毒，部分基因组测序显示它与植物呼肠孤病毒属的一种未分类病毒关系较远。另外还分离出两种已知病毒：库蚊Y病毒（双RNA病毒科）和休斯顿病毒（中病毒科）。我们还鉴定出了来自6个科（融合病毒科、双RNA病毒科、分节段病毒科、呼肠孤病毒科、番茄丛矮病毒科和双链RNA病毒科）的8种新型病毒序列、两种不属于任何已确立科的病毒以及许多先前已知的病毒。总之，我们提供了美国库蚊属蚊子中存在多种新型和已知病毒的确切证据。
