Blitvich Bradley J, Lin Ming, Dorman Karin S, Soto Victor, Hovav Einat, Tucker Bradley J, Staley Molly, Platt Kenneth B, Bartholomay Lyric C
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
J Med Entomol. 2009 Jul;46(4):934-41. doi: 10.1603/033.046.0428.
Adult mosquitoes (Diptera: Culicidae) were collected in 2007 and tested for specific viruses, including West Nile virus, as part of the ongoing arbovirus surveillance efforts in the state of Iowa. A subset of these mosquitoes (6,061 individuals in 340 pools) was further tested by reverse transcription-polymerase chain reaction (RT-PCR) using flavivirus universal primers. Of the 211 pools of Culex pipiens (L.) tested, 50 were positive. One of 51 pools of Culex tarsalis Coquillet was also positive. The flavivirus minimum infection rates (expressed as the number of positive mosquito pools per 1,000 mosquitoes tested) for Cx. pipiens and Cx. tarsalis were 10.3 and 1.2, respectively. Flavivirus RNA was not detected in Aedes triseriatus (Say) (52 pools), Culex erraticus (Dyar & Knab) (25 pools), or Culex territans Walker (one pool). Sequence analysis of all RT-PCR products revealed that the mosquitoes had been infected with Culex flavivirus (CxFV), an insect-specific virus previously isolated in Japan, Indonesia, Texas, Mexico, Guatemala and Trinidad. The complete genome of one isolate was sequenced, as were the envelope protein genes of eight other isolates. Phylogenetic analysis revealed that CxFV isolates from the United States (Iowa and Texas) are more closely related to CxFV isolates from Asia than those from Mexico, Guatemala, and Trinidad.
2007年采集了成年蚊子（双翅目：蚊科），并对包括西尼罗河病毒在内的特定病毒进行检测，作为爱荷华州正在进行的虫媒病毒监测工作的一部分。使用黄病毒通用引物，通过逆转录聚合酶链反应（RT-PCR）对这些蚊子的一个子集（340个样本池中的6061只个体）进行了进一步检测。在检测的211个尖音库蚊样本池中，50个呈阳性。在51个环跗库蚊样本池中，有1个也呈阳性。尖音库蚊和环跗库蚊的黄病毒最低感染率（以每检测1000只蚊子中阳性样本池的数量表示）分别为10.3和1.2。在三带喙库蚊（52个样本池）、迷走库蚊（25个样本池）或环斑库蚊（1个样本池）中未检测到黄病毒RNA。对所有RT-PCR产物的序列分析表明，这些蚊子感染了库蚊黄病毒（CxFV），这是一种先前在日本、印度尼西亚、德克萨斯州、墨西哥、危地马拉和特立尼达分离出的昆虫特异性病毒。对一个分离株的完整基因组进行了测序，另外八个分离株的包膜蛋白基因也进行了测序。系统发育分析表明，来自美国（爱荷华州和德克萨斯州）的CxFV分离株与来自亚洲的CxFV分离株的亲缘关系比与来自墨西哥、危地马拉和特立尼达的分离株更近。
