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上转换纳米颗粒平台用于高效树突状细胞抗原传递和同时追踪。

Upconversion nanoparticle platform for efficient dendritic cell antigen delivery and simultaneous tracking.

机构信息

Translational Nanobiomaterials and Imaging Group, Department of Radiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, the Netherlands.

Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands.

出版信息

Mikrochim Acta. 2022 Sep 3;189(10):368. doi: 10.1007/s00604-022-05441-z.

DOI:10.1007/s00604-022-05441-z
PMID:36057018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9440881/
Abstract

Upconversion nanoparticles (UCNPs) represent a group of NPs that can convert near-infrared (NIR) light into ultraviolet and visible light, thus possess deep tissue penetration power with less background fluorescence noise interference, and do not induce damage to biological tissues. Due to their unique optical properties and possibility for surface modification, UCNPs can be exploited for concomitant antigen delivery into dendritic cells (DCs) and monitoring by molecular imaging. In this study, we focus on the development of a nano-delivery platform targeting DCs for immunotherapy and simultaneous imaging. OVA 254-267 (OVA24) peptide antigen, harboring a CD8 T cell epitope, and Pam3CysSerLys4 (Pam3CSK4) adjuvant were chemically linked to the surface of UCNPs by amide condensation to stimulate DC maturation and antigen presentation. The OVA24-Pam3CSK4-UCNPs were thoroughly characterized and showed a homogeneous morphology and surface electronegativity, which promoted a good dispersion of the NPs. In vitro experiments demonstrated that OVA24-Pam3CSK4-UCNPs induced a strong immune response, including DC maturation, T cell activation, and proliferation, as well as interferon gamma (IFN-γ) production. In vivo, highly sensitive upconversion luminescence (UCL) imaging of OVA24-Pam3CSK4-UCNPs allowed tracking of UCNPs from the periphery to lymph nodes. In summary, OVA24-Pam3CSK4-UCNPs represent an effective tool for DC-based immunotherapy.

摘要

上转换纳米粒子（UCNPs）代表了一类能够将近红外（NIR）光转化为紫外和可见光的纳米粒子，因此具有深层组织穿透能力，背景荧光噪声干扰较小，且不会对生物组织造成损伤。由于其独特的光学性质和表面修饰的可能性，UCNPs 可用于同时将抗原递送至树突状细胞（DCs）并进行分子成像监测。在这项研究中，我们专注于开发一种针对 DCs 的纳米递药平台，用于免疫治疗和同时成像。OVA 254-267（OVA24）肽抗原，含有 CD8 T 细胞表位，以及 Pam3CysSerLys4（Pam3CSK4）佐剂，通过酰胺缩合化学连接到 UCNPs 的表面，以刺激 DC 成熟和抗原呈递。OVA24-Pam3CSK4-UCNPs 经过了全面的表征，显示出均匀的形态和表面电负性，这促进了 NPs 的良好分散。体外实验表明，OVA24-Pam3CSK4-UCNPs 诱导了强烈的免疫反应，包括 DC 成熟、T 细胞激活和增殖，以及干扰素γ（IFN-γ）的产生。在体内，对 OVA24-Pam3CSK4-UCNPs 的高灵敏度上转换发光（UCL）成像允许从外周追踪 UCNPs 到淋巴结。总之，OVA24-Pam3CSK4-UCNPs 代表了一种用于基于 DC 的免疫治疗的有效工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/6d082a19af8e/604_2022_5441_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/7a4637ba83f3/604_2022_5441_Sch1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/ee0f5cadb9ff/604_2022_5441_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/43fd3731d8e4/604_2022_5441_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/ea66fdcc094b/604_2022_5441_Fig4_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/19ea1ea92ea3/604_2022_5441_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/ca28b853d37e/604_2022_5441_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/6d082a19af8e/604_2022_5441_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/7a4637ba83f3/604_2022_5441_Sch1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/ee0f5cadb9ff/604_2022_5441_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/e6db7b9e11f0/604_2022_5441_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/43fd3731d8e4/604_2022_5441_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/ea66fdcc094b/604_2022_5441_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/fad988ccd7cb/604_2022_5441_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/19ea1ea92ea3/604_2022_5441_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/ca28b853d37e/604_2022_5441_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/029d/9440881/6d082a19af8e/604_2022_5441_Fig8_HTML.jpg

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1
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J Mater Chem C Mater. 2021 Nov 29;10(2):688-695. doi: 10.1039/d1tc04930e. eCollection 2022 Jan 7.
2
Functionalized Nanoparticles Targeting Tumor-Associated Macrophages as Cancer Therapy.靶向肿瘤相关巨噬细胞的功能化纳米颗粒用于癌症治疗
Pharmaceutics. 2021 Oct 13;13(10):1670. doi: 10.3390/pharmaceutics13101670.
3
Bioengineering of nano metal-organic frameworks for cancer immunotherapy.
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Front Bioeng Biotechnol. 2023 Oct 9;11:1242126. doi: 10.3389/fbioe.2023.1242126. eCollection 2023.
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Nano Res. 2021;14(5):1244-1259. doi: 10.1007/s12274-020-3179-9. Epub 2020 Nov 23.
4
Dendritic cell therapy in cancer treatment; the state-of-the-art.树突状细胞疗法在癌症治疗中的应用：最新进展。
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5
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6
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J Control Release. 2019 Dec 10;315:114-125. doi: 10.1016/j.jconrel.2019.10.048. Epub 2019 Oct 28.
7
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