Claude Emmanuelle, Towers Mark, Jones Emrys
Waters Corporation, Wilmslow, UK.
Methods Mol Biol. 2023;2688:41-54. doi: 10.1007/978-1-0716-3319-9_4.
Desorption electrospray ionization (DESI) is an ambient technique that allows chemical information to be obtained directly from a wide range of surfaces, without pretreatment. Here we describe the improvements that have been developed to be able to achieve low tens of microns pixel size MSI experiments with high sensitivity for metabolites and lipids from biological tissue sections.In the last decade, DESI mass spectrometry has undergone developmental improvements, with regard to the method of desorption and ionization as well as the mass spectrometer to which the DESI source has been coupled to. DESI is becoming a mass spectrometry imaging technique, which can match and complement the currently most widely adopted ionization technique, the matrix-assisted laser desorption/ionization (MALDI).
解吸电喷雾电离（DESI）是一种无需预处理就能直接从各种表面获取化学信息的常压技术。在此，我们描述了为实现低至几十微米像素尺寸的质谱成像（MSI）实验而进行的改进，该实验对生物组织切片中的代谢物和脂质具有高灵敏度。在过去十年中，DESI质谱在解吸和电离方法以及与DESI源联用的质谱仪方面都取得了发展改进。DESI正成为一种质谱成像技术，可与目前应用最广泛的电离技术——基质辅助激光解吸/电离（MALDI）相匹配并互补。
