School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, China.
National Center for International Research of Micro-Nano Molding Technology, Zhengzhou University, Zhengzhou 450001, China.
Int J Biol Macromol. 2022 Oct 31;219:1146-1154. doi: 10.1016/j.ijbiomac.2022.08.172. Epub 2022 Aug 31.
Surface modifications are common approaches to promote endothelialization and resist thrombus, therefore obtaining long-term patency of vascular grafts. Herein, we developed a functional coating based on hyaluronic acid (HA), dopamine (DA), and heparin. In the present study, dopamine was firstly grafted to the HA molecules. The DA-grafted HA material was then applied to the surface of fibrous PCL scaffold via oxidation polymerization. Heparin was directly loaded during the coating, instead of grafting. The composite coating enhanced the surface hydrophilicity of PCL scaffold, as well as the mechanical properties. Notably, the coated scaffold could promote EC proliferation and angiogenesis behavior via the upregulation of CD31 gene expression regardless of heparin addition. It also showed more effective inhibition of platelet adhesion and blood clotting in vitro. These results lead us to the conclusion that this functional coating is great potential in treating cardiovascular diseases in terms of promoting endothelialization, reducing thrombus, and maintaining the long-term patency of vascular grafts.
表面改性是促进内皮化和抗血栓形成、从而获得血管移植物长期通畅的常用方法。在此，我们开发了一种基于透明质酸（HA）、多巴胺（DA）和肝素的功能涂层。在本研究中，首先将多巴胺接枝到 HA 分子上。然后，通过氧化聚合将 DA 接枝的 HA 材料应用于纤维状聚己内酯（PCL）支架的表面。肝素在涂层过程中直接负载，而不是接枝。复合涂层增强了 PCL 支架的表面亲水性和力学性能。值得注意的是，涂层支架可通过上调 CD31 基因表达促进 EC 增殖和血管生成行为，而与肝素的添加无关。它在体外还表现出更有效的血小板黏附抑制和凝血抑制作用。这些结果使我们得出结论，这种功能涂层在促进内皮化、减少血栓形成和维持血管移植物的长期通畅方面，在治疗心血管疾病方面具有很大的潜力。
