Woestenborghs R J, Stanski D R, Scott J C, Heykants J J
Anesthesiology. 1987 Jul;67(1):85-90. doi: 10.1097/00000542-198707000-00015.
In this study, two independent laboratories assessed the validity of the fentanyl radioimmunoassay (RIA) by measuring a series of spiked control serum samples and 429 serum samples from 20 patients receiving fentanyl for their anesthesia. Additionally, a gas-liquid chromatographic (GLC) method specific for the parent drug was also applied to the same serum samples. The RIA measurement of fentanyl by the two laboratories resulted in comparable values for both control samples and samples from patients in a range of 0.5-50 ng/ml. The GLC method agreed with both RIA measurements in the spiked control and patient samples. The authors' results demonstrate the validity of the RIA as a measurement technique for fentanyl in human serum samples.
在本研究中，两个独立实验室通过检测一系列添加了芬太尼的对照血清样本以及来自20例接受芬太尼麻醉患者的429份血清样本，评估了芬太尼放射免疫分析（RIA）的有效性。此外，还将一种针对母体药物的气液色谱（GLC）方法应用于相同的血清样本。两个实验室对芬太尼的RIA检测结果显示，对照样本和患者样本在0.5 - 50 ng/ml范围内的值具有可比性。GLC方法与添加芬太尼的对照样本和患者样本的两种RIA检测结果均一致。作者的结果证明了RIA作为检测人血清样本中芬太尼的测量技术的有效性。
