Department of Ophthalmology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 200025, P. R. China.
Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, China.
BMC Ophthalmol. 2022 Sep 3;22(1):358. doi: 10.1186/s12886-022-02582-w.
Divided nevus with verrucous hyperplasia will always recur after surgery but non-verrucous divided eyelid nevus rarely recur. This study analyzed the differential expression of Ki-67, S100, Melan A and HMB45 and identified the correlation between the clinical and histopathological features of verrucous and non-verrucous divided eyelid nevus.
This study included 29 patients, of whom 8 patients had verrucous divided nevus. Immunohistochemistry labeling was used to assess the expression of Ki-67, S100, Melan A and HMB45 after excision. The difference between verrucous and non-verrucous divided eyelid nevus was analyzed.
The patients' ages ranged from 2 to 59 years, with a mean age of 19 years. The lesion size ranged from 1.5 to 2.0 cm in diameter and invaded the eyelid margins and the posterior lamella of the eyelids. Immunohistochemistry labeling showed strong positivity for approximately 98.5% of S100 and positive staining for approximately 27.6% of Ki-67, 72.4% of Melan A and 6.8% of HMB45. However, Ki-67 was significantly upregulated in verrucous divided nevus of the eyelids compared with non-verrucous divided nevus, with approximately 38.8% upregulation in verrucous and 18.3% upregulation in non-verrucous nevus.
This study correlated the clinic-pathologic features of verrucous divided eyelid nevus by means of statistically analyzing the varied clinical features and pathological impressions. The significant over-expression of S100 may be used as an indicator of divided nevus of the eyelids, and the over-expressed Ki-67 may contribute to the recurrence of verrucous divided nevus. High expression of HMB45 and Melan A may represent malignant transformation.
分裂痣伴疣状增生术后总会复发，但非疣状分裂性眼睑痣很少复发。本研究分析了 Ki-67、S100、Melan A 和 HMB45 的差异表达，并确定了疣状和非疣状分裂性眼睑痣的临床和组织病理学特征之间的相关性。
本研究纳入 29 例患者，其中 8 例为疣状分裂痣。切除后采用免疫组织化学标记法评估 Ki-67、S100、Melan A 和 HMB45 的表达。分析疣状和非疣状分裂性眼睑痣的差异。
患者年龄 259 岁，平均 19 岁。病变直径 1.52.0cm，侵犯眼睑边缘和眼睑后板。免疫组织化学标记显示 S100 阳性率约为 98.5%，Ki-67 阳性率约为 27.6%，Melan A 阳性率约为 72.4%，HMB45 阳性率约为 6.8%。然而，与非疣状分裂性眼睑痣相比，疣状分裂性眼睑痣的 Ki-67 表达明显上调，疣状痣上调约 38.8%，非疣状痣上调约 18.3%。
本研究通过对不同临床特征和病理印象进行统计学分析，对疣状分裂性眼睑痣的临床病理特征进行了相关性研究。S100 的显著过表达可能作为眼睑分裂痣的指标，Ki-67 的过表达可能导致疣状分裂性眼睑痣的复发。HMB45 和 Melan A 的高表达可能代表恶性转化。
