Department of Diagnosis and Surgery, São Paulo State University - UNESP, School of Dentistry at Araraquara, Araraquara, SP, Brazil; Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, School of Dentistry at Araraquara, São Paulo State University - UNESP, Araraquara, SP, Brazil.
Department of Diagnosis and Surgery, São Paulo State University - UNESP, School of Dentistry at Araçatuba, Araçatuba, SP, Brazil.
Arch Oral Biol. 2022 Nov;143:105529. doi: 10.1016/j.archoralbio.2022.105529. Epub 2022 Aug 22.
This study aimed to investigate polymorphisms in genes considered molecular biomarkers of type 2 diabetes mellitus (T2DM) to assess whether they are associated with periodontitis, and relating them to the periodontal status, glycemic and lipid profile of the subjects.
We investigated individuals who underwent complete periodontal examination and biochemical evaluation. We categorized them into three groups: (i) periodontitis with T2DM (Periodontitis+T2DM group, n = 206); (ii) periodontitis without T2DM (Periodontitis group, n = 346); and (iii) healthy individuals without Periodontitis or T2DM (Healthy group, n = 345). We investigated three single nucleotide polymorphisms (SNPs) for AGER, RBMS1 and VEGFA genes. We applied multivariate logistic and multiple linear regression models for all groups and stratified the subjects by sex and smoking habits.
Compared with RBMS1-rs7593730-CC+CT genotype carriers, RBMS1-rs7593730-TT carriers were more susceptible to periodontitis [odds ratio (OR) = 2.29; 95% confidence interval (CI) = 1.04-5.01; P-value = 0.033]. Among AGER-rs184003-CC carriers, never smokers had reduced risks of periodontitis and Periodontitis+T2DM than ever smokers. For either RBMS1-rs7593730-CC or VEGFA-rs9472138-CC carriers, never smokers had less susceptibility to develop periodontitis than ever smokers. Compared with AGER-rs184003-CC carriers, AGER-rs184003-AA carriers presented fewer remaining teeth. VEGFA-rs9472138-TT carriers showed a lower percentage of sites with characteristics of active periodontal disease (bleeding on pocket probing and interproximal clinical attachment level) compared with VEGFA-rs9472138-CC carriers.
In the studied population, AGER rs184003, RBMS1 rs7593730, and VEGFA rs9472138, which are considered genetic markers for T2DM, were associated with periodontitis without T2DM or periodontitis together with T2DM.
本研究旨在探讨被认为是 2 型糖尿病（T2DM）分子生物标志物的基因中的多态性，以评估它们是否与牙周炎相关，并将其与研究对象的牙周状况、血糖和血脂状况联系起来。
我们调查了接受完整牙周检查和生化评估的个体。我们将他们分为三组：（i）患有 T2DM 的牙周炎患者（牙周炎+T2DM 组，n=206）；（ii）无 T2DM 的牙周炎患者（牙周炎组，n=346）；和（iii）无牙周炎或 T2DM 的健康个体（健康组，n=345）。我们研究了三个单核苷酸多态性（SNP），用于AGER、RBMS1 和 VEGFA 基因。我们对所有组应用多元逻辑和多元线性回归模型，并按性别和吸烟习惯对受试者进行分层。
与 RBMS1-rs7593730-CC+CT 基因型携带者相比，RBMS1-rs7593730-TT 携带者更易患牙周炎[比值比（OR）=2.29；95%置信区间（CI）=1.04-5.01；P 值=0.033]。在AGER-rs184003-CC 携带者中，从不吸烟者患牙周炎和牙周炎+T2DM 的风险低于曾吸烟者。对于 RBMS1-rs7593730-CC 或 VEGFA-rs9472138-CC 携带者，从不吸烟者比曾吸烟者对牙周炎的易感性更低。与 AGER-rs184003-CC 携带者相比，AGER-rs184003-AA 携带者的余留牙更少。与 VEGFA-rs9472138-CC 携带者相比，VEGFA-rs9472138-TT 携带者的牙周袋探诊出血和邻面临床附着丧失等特征性活跃牙周病的位点比例较低。
在研究人群中，被认为是 T2DM 遗传标志物的AGER rs184003、RBMS1 rs7593730 和 VEGFA rs9472138 与无 T2DM 的牙周炎或与 T2DM 相关的牙周炎有关。
