Physiology Department, College of Medicine, King Saud University, Riyadh 11362, Saudi Arabia.
Deanship of Scientific Research, Umm Al-Qura University, Makkah 21961, Saudi Arabia.
Int J Mol Sci. 2023 Jul 11;24(14):11300. doi: 10.3390/ijms241411300.
Adipocytes play a critical role in maintaining a healthy systemic metabolism by storing and releasing energy in the form of fat and helping to regulate glucose and lipid levels in the body. Adipogenesis is the process through which pre-adipocytes are differentiated into mature adipocytes. It is a complex process involving various transcription factors and signaling pathways. The dysregulation of adipogenesis has been implicated in the development of obesity and metabolic disorders. Therefore, understanding the mechanisms that regulate adipogenesis and the factors that contribute to its dysregulation may provide insights into the prevention and treatment of these conditions. RNA-binding motif single-stranded interacting protein 1 (RBMS1) is a protein that binds to RNA and plays a critical role in various cellular processes such as alternative splicing, mRNA stability, and translation. RBMS1 polymorphism has been shown to be associated with obesity and type 2 diabetes, but the role of RBMS1 in adipose metabolism and adipogenesis is not known. We show that RBMS1 is highly expressed during the early phase of the differentiation of the murine adipocyte cell line 3T3-L1 and is significantly upregulated in the adipose tissue depots and adipocytes of high-fat-fed mice, implying a possible role in adipogenesis and adipose metabolism. Knockdown of RBMS1 in pre-adipocytes impacted the differentiation process and reduced the expression of some of the key adipogenic markers. Transcriptomic and proteomic analysis indicated that RBMS1 depletion affected the expression of several genes involved in major metabolic processes, including carbohydrate and lipid metabolism. Our findings imply that RBMS1 plays an important role in adipocyte metabolism and may offer novel therapeutic opportunity for metabolic disorders such as obesity and type 2 diabetes.
脂肪细胞在维持全身代谢健康方面起着关键作用，它们以脂肪的形式储存和释放能量，并有助于调节体内的葡萄糖和脂质水平。脂肪生成是前脂肪细胞分化为成熟脂肪细胞的过程。这是一个涉及多种转录因子和信号通路的复杂过程。脂肪生成的失调与肥胖和代谢紊乱的发展有关。因此，了解调节脂肪生成的机制以及导致其失调的因素，可能为这些疾病的预防和治疗提供新的思路。RNA 结合基序单链相互作用蛋白 1（RBMS1）是一种与 RNA 结合的蛋白质，在多种细胞过程中发挥着关键作用，如选择性剪接、mRNA 稳定性和翻译。RBMS1 多态性已被证明与肥胖和 2 型糖尿病有关，但 RBMS1 在脂肪代谢和脂肪生成中的作用尚不清楚。我们发现，RBMS1 在小鼠脂肪细胞系 3T3-L1 的分化早期高度表达，在高脂肪喂养小鼠的脂肪组织和脂肪细胞中显著上调，暗示其可能在脂肪生成和脂肪代谢中发挥作用。在预脂肪细胞中敲低 RBMS1 会影响分化过程，并降低一些关键脂肪生成标志物的表达。转录组学和蛋白质组学分析表明，RBMS1 耗竭会影响参与主要代谢过程的几个基因的表达，包括碳水化合物和脂质代谢。我们的研究结果表明，RBMS1 在脂肪细胞代谢中起着重要作用，可能为肥胖和 2 型糖尿病等代谢紊乱提供新的治疗机会。
