Department of Oral & Maxillofacial Surgery, Xiangya Stomatological Hospital & School of Stomatology, Central South University, Changsha, Hunan 410000, China; Hunan Key Laboratory of Oral Health Research, Xiangya School of Stomatology, Central South University, Changsha, Hunan 410000, China.
Department of Oral & Maxillofacial Surgery, Xiangya Stomatological Hospital & School of Stomatology, Central South University, Changsha, Hunan 410000, China; Hunan Key Laboratory of Oral Health Research, Xiangya School of Stomatology, Central South University, Changsha, Hunan 410000, China.
Arch Oral Biol. 2022 Nov;143:105519. doi: 10.1016/j.archoralbio.2022.105519. Epub 2022 Aug 18.
To study the expression, prognostic landscape, and the function of tubulin polymerization-promoting protein 3 (TPPP3) in oral squamous cell carcinoma (OSCC) and explore the relationship between TPPP3 expression and immune infiltration.
Immunohistochemical analysis using tissue microarray of 65 OSCC samples and 10 normal oral mucosa samples was performed to explore the expression levels in OSCC. 5 OSCC samples and 5 adjacent normal oral mucosa samples were selected to further analyze the expression levels by western blot. Some online bioinformatics analysis tools were adopted to study the transcriptome and prognostic landscape of TPPP3, the relationship of TPPP3 expression with immune infiltration, and the most possible "functions and pathways" of TPPP3 in head and neck squamous cell carcinoma (HNSC).
Immunohistochemical, western blot and bioinformatics analysis showed that the expression of TPPP3 in OSCC was significantly lower than that in the normal oral mucosa. High expression of TPPP3 was associated with a good prognosis of HNSC. TPPP3 expression was significantly positively correlated with infiltration level of B cell plasma and myeloid dendritic cells activated in HNSC, while it was negatively correlated with infiltration level of Tregs cells, CD8 T cells, B cell memory. Pathway analysis results revealed one most probable function and two significant pathways in which TPPP3 participates.
TPPP3 can serve as a potential diagnostic biomarker and a prognostic protective indicator of OSCC. It plays an important role in the recruitment and regulation of tumor-infiltrating immune cells, which may affect the survival of patients.
研究微管蛋白聚合促进蛋白 3（TPPP3）在口腔鳞状细胞癌（OSCC）中的表达、预后景观及其功能，并探讨 TPPP3 表达与免疫浸润的关系。
采用组织微阵列对 65 例 OSCC 样本和 10 例正常口腔黏膜样本进行免疫组织化学分析，以探讨 OSCC 中的表达水平。选择 5 例 OSCC 样本和 5 例相邻正常口腔黏膜样本进行进一步的western blot 分析，以分析 TPPP3 的表达水平。采用一些在线生物信息学分析工具，研究 TPPP3 的转录组和预后景观、TPPP3 表达与免疫浸润的关系，以及 TPPP3 在头颈部鳞状细胞癌（HNSC）中最可能的“功能和途径”。
免疫组化、western blot 和生物信息学分析表明，TPPP3 在 OSCC 中的表达明显低于正常口腔黏膜。TPPP3 的高表达与 HNSC 的良好预后相关。TPPP3 表达与 HNSC 中 B 细胞浆和髓系树突状细胞激活的浸润水平呈显著正相关，而与 Tregs 细胞、CD8 T 细胞、B 细胞记忆的浸润水平呈负相关。通路分析结果显示 TPPP3 参与了一个最可能的功能和两个重要的途径。
TPPP3 可作为 OSCC 的潜在诊断生物标志物和预后保护指标。它在募集和调节肿瘤浸润免疫细胞方面发挥着重要作用，可能影响患者的生存。
