A2b和A3腺苷受体可能在口腔鳞状细胞癌中充当促进因素和预后因素。

A2b and A3 adenosine receptors may act as promotive and prognostic factors in oral squamous cell carcinoma.

作者信息

Zeng Liujun, Wang Can, Wang Zijia, Xiong Haofeng, Zhang Tianyi, Chen Weijun, Hu Xin, Su Tong

机构信息

Department of Oral and Maxillofacial Surgery, Centre of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan, China; Research Center of Oral and Maxillofacial Tumor, Xiangya Hospital, Central South University, Changsha, Hunan, China; Institute of Oral Cancer and Precancerous Lesions, Central South University, China.

Department of Oral and Maxillofacial Surgery, Centre of Stomatology, Xiangya Hospital, Central South University, Changsha, Hunan, China; Department of Stomatology, Panyu Maternal and Child Care Service Centre, Guangzhou, Guangdong, China.

出版信息

J Craniomaxillofac Surg. 2023 Jul-Aug;51(7-8):508-515. doi: 10.1016/j.jcms.2023.08.002. Epub 2023 Aug 10.

DOI:10.1016/j.jcms.2023.08.002

PMID:37596174

Abstract

This study aimed to examine the expression and potential effects of adenosine receptors in oral squamous cell carcinoma (OSCC). Data on mRNA expression of adenosine receptors in OSCC samples were collected from The Cancer Genome Atlas database. Adenosine-regulated signaling pathways and biological processes were investigated via immune cell infiltration analysis, bioinformatics analysis, and immunohistochemistry. Overexpression of A2bR and A3R was significantly correlated with the prognosis of OSCC (P < 0.05). A3R expression in OSCC patients was significantly and positively correlated with the infiltration of six types of immune cells: B cells, CD4 T cells, CD8 T cells, neutrophils, macrophages, and myeloid dendritic cells. A2bR expression weakly and negatively correlated with B-cell infiltration only. The expression of A2bR in OSCC was positively correlated with E-cadherin and PCNA, while the expression of A3R was positively correlated with that of cleaved caspase-3. Within the limitations of the study it seems that the overexpression of A2bR and A3R results in the poor prognosis of OSCC, suggesting that A2bR promotes cell proliferation in OSCC, while A3R may be involved in OSCC progression by regulating tumor cell apoptosis and immune microenvironment.

摘要

本研究旨在检测腺苷受体在口腔鳞状细胞癌（OSCC）中的表达及潜在作用。从癌症基因组图谱数据库收集OSCC样本中腺苷受体的mRNA表达数据。通过免疫细胞浸润分析、生物信息学分析和免疫组织化学研究腺苷调节的信号通路和生物学过程。A2bR和A3R的过表达与OSCC的预后显著相关（P<0.05）。OSCC患者中A3R的表达与六种免疫细胞的浸润显著正相关：B细胞、CD4 T细胞、CD8 T细胞、中性粒细胞、巨噬细胞和髓样树突状细胞。A2bR的表达仅与B细胞浸润呈弱负相关。OSCC中A2bR的表达与E-钙黏蛋白和增殖细胞核抗原呈正相关，而A3R的表达与裂解的半胱天冬酶-3呈正相关。在本研究的局限性范围内，似乎A2bR和A3R的过表达导致OSCC预后不良，提示A2bR促进OSCC中的细胞增殖，而A3R可能通过调节肿瘤细胞凋亡和免疫微环境参与OSCC进展。