Institute of Postharvest and Food Science, Department of Postharvest Science, Volcani Institute, Agricultural Research Organization, Rishon LeZion 7505101, Israel; Institute of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Institute of Soil Water and Environmental Sciences, Volcani Institute, Agricultural Research Organization, POBox 6, Bet-Dagan 50250, Israel.
Biotechnol Adv. 2022 Dec;61:108031. doi: 10.1016/j.biotechadv.2022.108031. Epub 2022 Sep 1.
Recent studies highlight the therapeutic virtues of cannabidiol (CBD). Furthermore, due to their molecular enriched profiles, cannabis inflorescences are biologically superior to a single cannabinoid for the treatment of various health conditions. Thus, there is flourishing demand for Cannabis sativa varieties containing high levels of CBD. Additionally, legal regulations around the world restrict the cultivation and consumption of tetrahydrocannabinol (THC)-rich cannabis plants for their psychotropic effects. Therefore, the use of cannabis varieties that are high in CBD is permitted as long as their THC content does not exceed a low threshold of 0.3%-0.5%, depending on the jurisdiction. These chemovars are legally termed 'hemp'. This controlled cannabinoid requirement highlights the need to detect low levels of THC, already in the field. In this review, cannabis profiling and the existing methods used for the detection of cannabinoids are firstly evaluated. Then, selected valuable biosensor technologies are discussed, which suggest portable, rapid, sensitive, reproducible, and reliable methods for on-site identification of cannabinoids levels, mainly THC. Recent cutting-edge techniques of promising potential usage for both cannabis and hemp analysis are identified, as part of the future cultivation and agricultural improvement of this crop.
最近的研究强调了大麻二酚（CBD）的治疗功效。此外，由于其分子丰富的特征，与单一大麻素相比，大麻花序在治疗各种健康状况方面具有生物学优势。因此，对含有高水平 CBD 的大麻品种的需求正在蓬勃增长。此外，由于四氢大麻酚（THC）含量高的大麻植物具有致幻作用，世界各地的法律规定都限制其种植和消费。因此，只要其 THC 含量不超过 0.3%-0.5%的低阈值（具体取决于司法管辖区），就允许使用 CBD 含量高的大麻品种。这些化学变种在法律上被称为“大麻”。这种对受控大麻素的要求凸显了在田间检测低水平 THC 的必要性。在这篇综述中，首先评估了大麻分析和现有的大麻素检测方法。然后，讨论了选定的有价值的生物传感器技术，这些技术为现场鉴定大麻素水平（主要是 THC）提供了便携、快速、灵敏、可重复和可靠的方法。作为该作物未来种植和农业改良的一部分，确定了一些有前途的潜在用于大麻和大麻分析的最新前沿技术。
