Research Center of Neurology, Moscow, Russia.
M. V. Lomonosov Moscow State University, Moscow, Russia.
Bull Exp Biol Med. 2022 Aug;173(4):539-543. doi: 10.1007/s10517-022-05578-0. Epub 2022 Sep 5.
Addition of 0.07 mM zinc ions to the culture medium induced death of cerebellar granule neurons. This was preceded by a decrease in intracellular pH by 0.86±0.12 and an increase in the level of intracellular zinc registered by increasing fluorescence of FluoZin3. However, intracellular acidosis caused by acidification of the culture medium to pH 6.0 was not toxic to neurons, and even significantly increased their survival under the action of zinc ions. In general, the overload of the neurons cytoplasm with zinc ions causes acidification of the cytoplasm, which is probably associated with the activation of Zn2/H exchangers and is a protective mechanism for the neurocytotoxic effect of zinc ions.
向培养基中添加 0.07 mM 的锌离子会诱导小脑颗粒神经元死亡。这是由细胞内 pH 值下降 0.86±0.12 和 FluoZin3 荧光强度增加所记录到的细胞内锌水平升高所引起的。然而，将培养基酸化至 pH 6.0 引起的细胞内酸中毒对神经元并无毒性，甚至在锌离子的作用下显著增加了神经元的存活率。总的来说，锌离子使神经元细胞质超载会导致细胞质酸化，这可能与 Zn2+/H+交换器的激活有关，是锌离子神经细胞毒性作用的一种保护机制。
