Research Center of Neurology, Moscow, Russia.
Lomonosov Moscow State University, Moscow, Russia.
Bull Exp Biol Med. 2024 Sep;177(5):588-591. doi: 10.1007/s10517-024-06229-2. Epub 2024 Sep 28.
We studied the effect of extracellular acidosis, cysteine, glutathione, and iron ions (Fe) on the neurocytotoxic effect of copper ions (Cu) in vitro. At acidic pH of the culture medium (pH 6.8), the toxic effect of copper on cultured neurons significantly increased in comparison with that at neutral pH 7.3. In the presence of 25 μM Cu in the culture medium at pH 7.3 and 6.8, the neuronal survival was 89±2 and 63±4%, respectively. In the presence of glutathione or cysteine (1 μM) in the culture medium, even 0.5 μM Cu caused 100% death of cultured neurons, while the presence of Fe (10-50 μM) had no effect on the toxicity of Cu. In general, acidosis or the presence of glutathione or cysteine increases the cytotoxicity of copper ions.
我们研究了细胞外酸中毒、半胱氨酸、谷胱甘肽和铁离子（Fe）对铜离子（Cu）在体外的神经细胞毒性的影响。在培养基的酸性 pH 值（pH 6.8）下，与中性 pH 值 7.3 相比，铜对培养神经元的毒性作用明显增加。在 pH 值为 7.3 和 6.8 的培养基中存在 25 μM Cu 的情况下，神经元的存活率分别为 89±2%和 63±4%。在培养基中存在谷胱甘肽或半胱氨酸（1 μM）的情况下，即使存在 0.5 μM Cu 也会导致培养神经元 100%死亡，而铁（10-50 μM）的存在对 Cu 的毒性没有影响。总的来说，酸中毒或谷胱甘肽或半胱氨酸的存在增加了铜离子的细胞毒性。
