Choi Sujin, Lee Soonchul, Han Young-Hoon, Choi Junwon, Kim Isaac, Lee Jusung, An Hyun-Ju
Department of Orthopaedic Surgery, CHA Bundang Medical Center, CHA University School of Medicine, Pangyo-ro, South Korea.
Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul, South Korea.
Front Oncol. 2022 Aug 18;12:945057. doi: 10.3389/fonc.2022.945057. eCollection 2022.
MicroRNAs are key regulators of gene expression in tumorigenesis. In this study, we investigated the tumor-suppressive function of miR-31-3p. Analysis of the Gene Expression Omnibus database revealed that the expression of miR-31-3p in prostate cancer tissues is lower than that in adjacent normal tissues from patients with prostate cancer. Moreover, miR-31-3p induces apoptosis in DU145, PC-3, and LNCap prostate cancer cells, while those transfected with miR-31-3p exhibit significantly decreased cell proliferation, migration, invasiveness, and tumor sphere-forming ability, as determined using the cell counting kit-8, transwell, and sphere-forming assays. Further analysis revealed that GABBR2 is a direct target of miR-31-3p. Within a DU145 xenograft murine model, intratumoral injection of a miR-31-3p mimic suppresses tumor growth. Taken together, the findings of this study suggest that miR-31-3p performs a novel tumor-suppressive function in prostate cancer and may represent a novel target for anti-prostate cancer miRNA therapeutics.
微小RNA是肿瘤发生过程中基因表达的关键调节因子。在本研究中，我们调查了miR-31-3p的肿瘤抑制功能。对基因表达综合数据库的分析显示，前列腺癌组织中miR-31-3p的表达低于前列腺癌患者的相邻正常组织。此外，miR-31-3p可诱导DU145、PC-3和LNCap前列腺癌细胞凋亡，而用miR-31-3p转染的细胞表现出细胞增殖、迁移、侵袭和肿瘤球形成能力显著降低，这是通过细胞计数试剂盒-8、Transwell和球形成试验确定的。进一步分析表明，GABBR2是miR-31-3p的直接靶点。在DU145异种移植小鼠模型中，瘤内注射miR-31-3p模拟物可抑制肿瘤生长。综上所述，本研究结果表明，miR-31-3p在前列腺癌中发挥新的肿瘤抑制功能，可能代表抗前列腺癌miRNA治疗的新靶点。
