Xu Yanglong, Zou Xue, Mei Jie
Department of General Dentistry, Stomatological Hospital Affiliated to Zunyi Medical University, China.
Department of Periodontics, Stomatological Hospital Affiliated to Zunyi Medical University, China.
Appl Bionics Biomech. 2022 Aug 24;2022:1666792. doi: 10.1155/2022/1666792. eCollection 2022.
N7-methylguanosine modification-related lncRNAs (m7G-related lncRNAs) are involved in progression of many diseases. This study was aimed at revealing the risk correlation between N7-methylguanosine modification-related lncRNAs and survival prognosis of oral squamous cell carcinoma.
In the present study, coexpression network analysis and univariate Cox analysis were used to obtained 31 m7G-related mRNAs and 399 m7G-related lncRNAs. And the prognostic risk score model of OSCC patients was evaluated and optimized through cross-validation.
Through the coexpression analysis and risk assessment analysis of m7G-related prognostic mRNAs and lncRNAs, it was found that six m7G-related prognostic lncRNAs (AC005332.6, AC010894.1, AC068831.5, AL035446.1, AL513550.1, and HHLA3) were high-risk lncRNAs. Three m7G-related prognostic lncRNAs (AC007114.1, HEIH, and LINC02541) were protective lncRNAs. Then, survival curves were drawn by comparing the survival differences between patients with high and low expression of each m7G-related prognostic lncRNA in the prognostic risk score model. Further, risk curves, scatter plots, and heat maps were drawn by comparing the survival differences between high-risk and low-risk OSCC patients in the prognostic model. Finally, forest maps and the ROC curve were generated to verify the predictive power of the prognostic risk score model. Our results will help to find early and accurate prognostic risk markers for OSCC, which could be used for early prediction and early clinical intervention of survival, prognosis, and disease risk of OSCC patients in the future.
N7-甲基鸟苷修饰相关长链非编码RNA（m7G相关lncRNAs）参与多种疾病的进展。本研究旨在揭示N7-甲基鸟苷修饰相关lncRNAs与口腔鳞状细胞癌生存预后之间的风险相关性。
在本研究中，采用共表达网络分析和单因素Cox分析获得31个m7G相关mRNA和399个m7G相关lncRNAs。并通过交叉验证对OSCC患者的预后风险评分模型进行评估和优化。
通过对m7G相关预后mRNA和lncRNAs的共表达分析和风险评估分析，发现6个m7G相关预后lncRNAs（AC005332.6、AC010894.1、AC068831.5、AL035446.1、AL513550.1和HHLA3）为高危lncRNAs。3个m7G相关预后lncRNAs（AC007114.1、HEIH和LINC02541）为保护性lncRNAs。然后，通过比较预后风险评分模型中各m7G相关预后lncRNA高表达和低表达患者的生存差异绘制生存曲线。此外，通过比较预后模型中高危和低危OSCC患者的生存差异绘制风险曲线、散点图和热图。最后，生成森林图和ROC曲线以验证预后风险评分模型的预测能力。我们的结果将有助于找到OSCC早期准确的预后风险标志物，可用于未来OSCC患者生存、预后和疾病风险的早期预测和早期临床干预。
