Characterization and evaluation of anti- activity of indigenous probiotic lactobacilli in mice.

Lactobacilli ( = 24), isolated from human infants and yogurt, showed variable activity against (8.0 ± 1.0 to 16.6 ± 0.5 mm) and other gut pathogens (9.0 ± 1.0 to 15.3 ± 0.5 mm), as determined by a well diffusion assay. The isolates were identified as (FY1, FY3, FY4, IL2, and IL5), (FY6 and FY7), sp. (IL7), and (IL12). All isolates showed variable tolerance to acidic pH for 3 h and visible growth at pH 4 and in the presence of 0.3% ox-bile. The antibiotic susceptibility profile of isolates indicated resistance against vancomycin, ciprofloxacin, streptomycin, and lincomycin. Isolates had variable auto-aggregation and showed variable capabilities to co-aggregate with . Based on all tested parameters, IL2, IL5, and IL12 were selected for co-culture experiments, followed by evaluation in Balb/c mice. All the selected isolates resulted in a 100% reduction in in broth. isolates efficiently colonized mouse guts and inhibited colonization. Overall, there was ≥99.06% and ≤4.32 Mean log reduction in counts in mice feces within 7 days. The study, thus, provided characterized lactobacilli that could be considered as potential ingredients for probiotic formulations intended to prevent infection in humans.