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新型菌株在禽业中的应用及其抗沙门氏菌潜力

Anti-Salmonella Potential of New Strains with the Application in the Poultry Industry.

作者信息

Kowalska Justyna D, Nowak Adriana, Śliżewska Katarzyna, Stańczyk Małgorzata, Łukasiak Magdalena, Dastych Jarosław

机构信息

Proteon Pharmaceuticals SA , Lodz , Poland.

Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology , Lodz , Poland.

出版信息

Pol J Microbiol. 2020 Sep;69(1):5-18. doi: 10.33073/pjm-2020-001. Epub 2020 Jan 28.

Abstract

Probiotics are considered an alternative to antibiotics in the prevention and treatment of diseases in poultry. However, to use probiotics as proposed above, it is necessary to evaluate their properties in detail and to select the most effective bacterial strains in the application targeted. In this study, probiotic properties of new sp. strains were investigated and their antimicrobial activity against 125 environmental strains of sp. was determined using the agar slab method. Furthermore, their survival in the presence of bile salts and at low pH, antibiotics susceptibility, aggregation and coaggregation ability, adherence to polystyrene and Caco-2 cells, and cytotoxicity were investigated. Each strain tested showed antagonistic activity against at least 96% of the environmental sp. strains and thus representing a highly epidemiologically differentiated collection of poultry isolates. In addition, the probiotic properties of new strains are promising. Therefore, all strains examined showed a high potential for use in poultry against salmonellosis. Probiotics are considered an alternative to antibiotics in the prevention and treatment of diseases in poultry. However, to use probiotics as proposed above, it is necessary to evaluate their properties in detail and to select the most effective bacterial strains in the application targeted. In this study, probiotic properties of new sp. strains were investigated and their antimicrobial activity against 125 environmental strains of sp. was determined using the agar slab method. Furthermore, their survival in the presence of bile salts and at low pH, antibiotics susceptibility, aggregation and coaggregation ability, adherence to polystyrene and Caco-2 cells, and cytotoxicity were investigated. Each strain tested showed antagonistic activity against at least 96% of the environmental sp. strains and thus representing a highly epidemiologically differentiated collection of poultry isolates. In addition, the probiotic properties of new strains are promising. Therefore, all strains examined showed a high potential for use in poultry against salmonellosis.

摘要

益生菌被认为是家禽疾病预防和治疗中抗生素的替代品。然而,要按上述提议使用益生菌,有必要详细评估其特性,并在目标应用中选择最有效的菌株。在本研究中,对新的sp.菌株的益生菌特性进行了研究,并使用琼脂平板法测定了它们对125株sp.环境菌株的抗菌活性。此外,还研究了它们在胆盐存在下和低pH值环境中的存活情况、抗生素敏感性、聚集和共聚集能力、对聚苯乙烯和Caco-2细胞的粘附性以及细胞毒性。每个测试菌株对至少96%的环境sp.菌株都表现出拮抗活性,因此代表了一组高度流行病学分化的家禽分离株。此外,新菌株的益生菌特性很有前景。因此,所有检测菌株在用于家禽防治沙门氏菌病方面都显示出很高的潜力。益生菌被认为是家禽疾病预防和治疗中抗生素的替代品。然而,要按上述提议使用益生菌,有必要详细评估其特性,并在目标应用中选择最有效的菌株。在本研究中,对新的sp.菌株的益生菌特性进行了研究,并使用琼脂平板法测定了它们对125株sp.环境菌株的抗菌活性。此外,还研究了它们在胆盐存在下和低pH值环境中的存活情况、抗生素敏感性、聚集和共聚集能力、对聚苯乙烯和Caco-2细胞的粘附性以及细胞毒性。每个测试菌株对至少96%的环境sp.菌株都表现出拮抗活性,因此代表了一组高度流行病学分化的家禽分离株。此外,新菌株的益生菌特性很有前景。因此,所有检测菌株在用于家禽防治沙门氏菌病方面都显示出很高的潜力。

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