Ronning I E, Frank H A
Appl Environ Microbiol. 1987 May;53(5):1020-7. doi: 10.1128/aem.53.5.1020-1027.1987.
The inhibitory effects of potassium sorbate on the bioenergetics, phenylalanine uptake, protein synthesis, and certain aspects of cell regulation were examined in putrefactive anaerobe 3679. Undissociated sorbic acid appeared to act as a protonophore by lowering the intracellular pH and dissipating the proton motive force of the membrane. Sorbate inhibited the uptake of phenylalanine, decreased the rate of protein synthesis, and altered patterns of phosphorylated nucleotide accumulation, resulting in increased intracellular concentrations of GTP, ppGpp, and an unidentified compound (possibly pppGpp). The addition of a noninhibitory amount of tetracycline released the inhibition of growth by sorbate. Based on these results, we concluded that the inhibition of putrefactive anaerobe 3679 by sorbate resulted from a stringent-type regulatory response induced by the protonophoric activity of sorbic acid.
研究了山梨酸钾对腐败厌氧菌3679生物能量学、苯丙氨酸摄取、蛋白质合成及细胞调节某些方面的抑制作用。未解离的山梨酸似乎通过降低细胞内pH值和耗散膜的质子动力而起质子载体的作用。山梨酸盐抑制苯丙氨酸的摄取，降低蛋白质合成速率，并改变磷酸化核苷酸积累模式，导致细胞内GTP、ppGpp和一种未鉴定化合物（可能是pppGpp）浓度增加。添加非抑制量的四环素可解除山梨酸盐对生长的抑制。基于这些结果，我们得出结论，山梨酸盐对腐败厌氧菌3679的抑制作用是由山梨酸的质子载体活性诱导的严紧型调节反应所致。
