使用提取物进行银纳米颗粒的气相色谱/质谱分析及植物合成：抗菌、抗氧化作用、抗癌及凋亡作用
GC/MS Analysis and Phyto-synthesis of Silver Nanoparticles Using Extract: Antibacterial, Antioxidant Effects, Anticancer and Apoptotic Effects.
作者信息
Farmahini Farahani Azadeh, Hamdi Seyed Mohammad Mahdi, Mirzaee Amir
机构信息
Department of Biology, Central Tehran branch, Islamic Azad University, Tehran, Iran.
Department of Biology, Roudehen branch, Islamic Azad University, Roudehen, Iran.
出版信息
Avicenna J Med Biotechnol. 2022 Jul-Sep;14(3):223-232. doi: 10.18502/ajmb.v14i3.9829.
BACKGROUND
The present study was aimed at phyto-synthesized silver nanoparticles (AgNPs) using ( extract and to investigate the antibacterial, antioxidant effects, anticancer and apoptotic effects of phyto-synthesized AgNPs.
METHODS
The bio-fabricated AgNPs were characterized using UV-visible spectroscopy (UV-visible), X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR), Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and Energy Disper-sive X-ray (EDX).
RESULTS
The phyto-synthesized AgNPs showed maximum absorption in 438 , in the UV-visible spectrum. XRD peaks were observed at 2θ values in 38.20°, 44.40°, 64.60°, and 77.50° which are indexed as (111), (200), (220), and (311) bands of Face-Centered Cubic (FCC) structures of silver. FTIR analysis indicated that the AgNPs were capped with extract. SEM and TEM micrographs revealed that the fabricated AgNPs were spherical and the average size range was 17.89 . Also, the EDX results show that the content of Ag was 90%.
CONCLUSION
The phyto-synthesized AgNPs had significant antibacterial activity against Gram-negative bacteria, as well as, the AgNPs exhibited great inhibitory effects on DPPH radicals and their antioxidant properties were favorably comparable to the antioxidant outcomes of ascorbic acid. Moreover, the AgNPs showed anti-cancer activity against the MCF-7 cell line with the IC50=6.1 . Moreover, the phyto-synthesized AgNPs could induce apoptosis in the MCF-7 cell line significantly. The GC-MS analysis of the extract showed that 102 bioactive phyto-chemical compounds, which be of use to the synthesis of AgNPs.
背景
本研究旨在利用（提取物）生物合成银纳米颗粒（AgNPs），并研究生物合成的AgNPs的抗菌、抗氧化、抗癌及凋亡作用。
方法
利用紫外可见光谱（UV-visible）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）和能量色散X射线光谱（EDX）对生物合成的AgNPs进行表征。
结果
生物合成的AgNPs在紫外可见光谱中于438 处显示出最大吸收峰。在2θ值为38.20°、44.40°、64.60°和77.50°处观察到XRD峰，这些峰对应于银的面心立方（FCC）结构的（111）、（200）、（220）和（311）晶带。FTIR分析表明AgNPs被（提取物）包覆。SEM和TEM显微照片显示，合成的AgNPs呈球形，平均尺寸范围为17.89 。此外，EDX结果表明Ag的含量为90%。
结论
生物合成的AgNPs对革兰氏阴性菌具有显著的抗菌活性，并且AgNPs对DPPH自由基表现出很强的抑制作用，其抗氧化性能与抗坏血酸的抗氧化效果相当。此外，AgNPs对MCF-7细胞系具有抗癌活性，IC50 = 6.1 。而且，生物合成的AgNPs可显著诱导MCF-7细胞系凋亡。对（提取物）的GC-MS分析表明，有102种生物活性植物化学化合物可用于AgNPs的合成。
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