School of Chemistry and Chemical Engineering, North University of China, Taiyuan 030051, China.
Molecules. 2023 Sep 4;28(17):6431. doi: 10.3390/molecules28176431.
The present study reports the biomimetic synthesis of silver nanoparticles (AgNPs) using a simple, cost effective and eco-friendly method. In this method, the flavonoid extract of (PFFE) was used as a bioreduction agent for the reduction of metallic silver into nanosilver, called flavonoid extract silver nanoparticles (PFFE-AgNPs). The Ultraviolet-Visible (UV-Vis) spectrum showed a characteristic absorption peak at 440 nm that confirmed the synthesis of PFFE-AgNPs. A Fourier transform infrared spectroscopic (FTIR) analysis of the PFFE-AgNPs revealed that flavonoids are involved in the bioreduction and capping processes. X-ray diffraction (XRD) and selected area electron diffraction (SAED) patterns confirmed the face-centered cubic (FCC) crystal structure of PFFE-AgNPs. A transmission electron microscopic (TEM) analysis indicated that the synthesized PFFE-AgNPs are 20 to 70 nm in size with spherical morphology and without any aggregation. Dynamic light scattering (DLS) studies showed that the average hydrodynamic size was 44 nm. A polydispersity index (PDI) of 0.321 denotes the monodispersed nature of PFFE-AgNPs. Further, a highly negative surface charge or zeta potential value (-30 mV) indicates the repulsion, non-aggregation, and stability of PFFE-AgNPs. PFFE-AgNPs showed cytotoxic effects against cancer cell lines, including human colon carcinoma (COLO205) and mouse melanoma (B16F10), with IC concentrations of 59.57 and 69.33 μg/mL, respectively. PFFE-AgNPs showed a significant inhibition of both Gram-positive ( and ) and Gram-negative ( and ) bacteria pathogens. PFFE-AgNPs exhibited in vitro antioxidant activity by quenching 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide (HO) free radicals with IC values of 72.81 and 92.48 µg/mL, respectively. In this study, we also explained the plausible mechanisms of the biosynthesis, anticancer, and antibacterial effects of PFFE-AgNPs. Overall, these findings suggest that PFFE-AgNPs have potential as a multi-functional nanomaterial for biomedical applications, particularly in cancer therapy and infection control. However, it is important to note that further research is needed to determine the safety and efficacy of these nanoparticles in vivo, as well as to explore their potential in other areas of medicine.
本研究报告了使用简单、经济实惠且环保的方法仿生合成银纳米粒子（AgNPs）。在该方法中，使用（PFFE）的类黄酮提取物作为生物还原剂，将金属银还原为纳米银，称为类黄酮提取物银纳米粒子（PFFE-AgNPs）。紫外-可见（UV-Vis）光谱在 440nm 处显示出特征吸收峰，证实了 PFFE-AgNPs 的合成。对 PFFE-AgNPs 的傅里叶变换红外光谱（FTIR）分析表明，类黄酮参与了生物还原和封端过程。X 射线衍射（XRD）和选区电子衍射（SAED）图谱证实了 PFFE-AgNPs 的面心立方（FCC）晶体结构。透射电子显微镜（TEM）分析表明，合成的 PFFE-AgNPs 尺寸为 20 至 70nm，具有球形形态且无任何聚集。动态光散射（DLS）研究表明，平均水动力尺寸为 44nm。多分散指数（PDI）为 0.321 表示 PFFE-AgNPs 的单分散性质。此外，高负表面电荷或 zeta 电位值（-30mV）表明 PFFE-AgNPs 的排斥、非聚集和稳定性。PFFE-AgNPs 对包括人结肠癌细胞（COLO205）和小鼠黑色素瘤（B16F10）在内的癌细胞系表现出细胞毒性作用，IC 浓度分别为 59.57 和 69.33μg/mL。PFFE-AgNPs 对革兰氏阳性（和）和革兰氏阴性（和）细菌病原体均表现出显著的抑制作用。PFFE-AgNPs 通过猝灭 1,1-二苯基-2-苦基肼（DPPH）和过氧化氢（HO）自由基表现出体外抗氧化活性，IC 值分别为 72.81 和 92.48μg/mL。在这项研究中，我们还解释了 PFFE-AgNPs 的生物合成、抗癌和抗菌作用的可能机制。总的来说，这些发现表明 PFFE-AgNPs 具有作为生物医学应用多功能纳米材料的潜力，特别是在癌症治疗和感染控制方面。然而，需要注意的是，还需要进一步的研究来确定这些纳米粒子在体内的安全性和有效性，以及探索它们在医学其他领域的潜在应用。
