Nasrollahi Saman Ahmad, Fattahi Mahsa, Khamesipoor Ali, Amiri Fatemeh, Ahmadi Maryam, Kavkani Mahshid Shahrzad, Lotfali Ensieh, Ayatollahi Azin, Skandari Seyed Ebrahim, Firooz Alireza
Drs. Nasrollahi, Fattahi, Khamesipoor, Amiri, Ahmadi, Ayatollahi, Skandari, and Firooz are with the Center for Research and Training in Skin Diseases and Leprosy at Tehran University of Medical Sciences in Tehran, Iran.
Mrs. Kavkani is with the Department of Science at Damghan Branch at Islamic Azad University in Damghan, Iran.
J Clin Aesthet Dermatol. 2022 Aug;15(8):34-37.
The present study was designed to evaluate the effects of seven common preservatives used in Iranian cosmetic products on facial skin microflora.
Fifteen healthy volunteers, aged 20 to 35 years, were recruited. Three symmetrical sites from the cheeks of each volunteer were selected and samples were collected. DNA was extracted from the culture using the boiling method. The fungi's internal transcribed spacer (ITS) region was amplified using ITS1/ITS4 primers, for 16s to identify bacteria and specific primers. The effects of the preservatives were assessed based on growth on broth culture media.
Primary identification was based on yeast on CHROM agar, in which 15 different yeasts were isolated; then, PCR was used to identified the species as: (n: 14; 93%), (n: 1; 7%). One primary identified yeast on Dixon media was precisely differentiated as using the PCR method. Fifteen primary identified cocci on tryptic soy agar media were identified as . All the preservatives showed to inhibit the growth of isolated fungi, but not that of bacterial microflora.
The present study showed preservatives in cosmetic products can alter skin microflora while also preventing the growth of pathogenic bacteria.
本研究旨在评估伊朗化妆品中使用的七种常见防腐剂对面部皮肤微生物群的影响。
招募了15名年龄在20至35岁之间的健康志愿者。从每位志愿者脸颊上选取三个对称部位并采集样本。使用煮沸法从培养物中提取DNA。使用ITS1/ITS4引物扩增真菌的内部转录间隔区（ITS）区域，使用16s特异性引物鉴定细菌。根据肉汤培养基上的生长情况评估防腐剂的效果。
初步鉴定基于CHROM琼脂上的酵母，从中分离出15种不同的酵母；然后，使用PCR将菌种鉴定为：（n：14；93%），（n：1；7%）。使用PCR方法将Dixon培养基上一种初步鉴定的酵母精确鉴定为。在胰蛋白胨大豆琼脂培养基上初步鉴定的15种球菌被鉴定为。所有防腐剂均显示出抑制分离出的真菌生长的作用，但对细菌微生物群无此作用。
本研究表明，化妆品中的防腐剂可改变皮肤微生物群，同时还能抑制病原菌的生长。
