载有表没食子儿茶素没食子酸酯的黏膜黏附性牙龈贴片对牙周炎中白细胞介素-6和白细胞介素-10表达的影响。
The ability of mucoadhesive gingival patch loaded with EGCG on IL-6 and IL-10 expression in periodontitis.
作者信息
Lashari Dur M, Aljunaid Mohammed Ahmed, Ridwan Rini Devijanti, Diyatri Indeswati, Lashari Yasmeen, Qaid Huda, Surboyo Meircurius Dwi Condro
机构信息
Doctoral Program of Dental Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Dentistry Faculty of Medicine, University of Taiz, Taiz, Yemen.
出版信息
J Oral Biol Craniofac Res. 2022 Sep-Oct;12(5):679-682. doi: 10.1016/j.jobcr.2022.08.007. Epub 2022 Aug 23.
OBJECTIVES
The mucoadhesive gingival patch is a topical drug delivery process it does not cause any irritation in the mucosa. EGCG (Epigallocatechin-3-gallate) it has potent antioxidant, antiangiogenic and antitumor effects. The present study investigates the ability of mucoadhesive gingival patch loaded with EGCG on periodontitis and its impact on IL-6 and IL-10 expression.
METHOD
Periodontitis model was developed in Wistar rat by induction of . Application of mucoadhesive gingival patch loaded with EGCG (GP-EGCG), mucoadhesive gingival patch loaded with doxycycline (GP-doxy) and blank patch, was done for treated periodontitis 1 h each day during 21 days. Indirect immunohistochemical analysis of IL-6 and IL-10 expressions were analyzed in the mandibular preparation of the anterior incisive region of animal.
RESULTS
The GP-EGCG treatment for 3 days until 21 days, consistently increased the IL-10 expression in periodontitis (p < 0.05). In other hand, GP-EGCG treatment lowered the IL-6 expression after 7, 14 and 21 days (p < 0.05).
CONCLUSION
The GP-EGCG is promising for the periodontitis treatment by interfere the IL-6 and IL-10 expression.
目的
黏膜黏附性牙龈贴片是一种局部给药方法，不会对黏膜造成任何刺激。表没食子儿茶素没食子酸酯（EGCG）具有强大的抗氧化、抗血管生成和抗肿瘤作用。本研究调查了载有EGCG的黏膜黏附性牙龈贴片对牙周炎的治疗能力及其对白细胞介素-6（IL-6）和白细胞介素-10（IL-10）表达的影响。
方法
通过诱导在Wistar大鼠中建立牙周炎模型。对治疗牙周炎的大鼠，每天1小时，持续21天，分别应用载有EGCG的黏膜黏附性牙龈贴片（GP-EGCG）、载有强力霉素的黏膜黏附性牙龈贴片（GP-强力霉素）和空白贴片。对动物下颌前切牙区域制备物中的IL-6和IL-10表达进行间接免疫组织化学分析。
结果
GP-EGCG治疗3天至21天，持续增加牙周炎中IL-10的表达（p<0.05）。另一方面，GP-EGCG治疗7天、14天和21天后降低了IL-6的表达（p<0.05）。
结论
GP-EGCG有望通过干扰IL-6和IL-10的表达来治疗牙周炎。
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