Division of Viral Hepatitis, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, 30329, USA.
Department of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo, 113-8519, Japan.
BMC Res Notes. 2022 Sep 5;15(1):287. doi: 10.1186/s13104-022-06178-x.
Use of dried blood spots (DBS) for detection of hepatitis B virus (HBV) markers of infection has the potential to facilitate diagnosis of HBV infection especially in resource-limited countries. The aim of this study was to evaluate the feasibility of DBS for detection of various markers of HBV infections.
Fifty-four DBS samples were engineered from well-characterized plasma samples. All DBS samples were tested for HBsAg, total anti-HBc and HBV DNA, 20 of 54 samples were also tested for HBeAg using commercially available assays. HBsAg was detected in 24 of 25 (96%), HBV DNA in 22 of 25 (88%), total anti-HBc in all 9 (100%), and HBeAg in all 7 (100%) DBS samples. The average difference in HBV DNA levels between DBS eluates and corresponding plasma samples was 2.7 log IU/mL. Fifteen DBS eluates positive for HBV DNA were sequenced and all of them belonged to HBV genotype A. Thirteen samples which were negative for all HBV markers showed HBeAg false positivity. Therefore, DBS is a reliable sample matrix for detection of HBsAg, total anti-HBc and HBV DNA, but not HBeAg. Further feasibility studies of DBS for diagnostic purposes and epidemiologic studies are warranted.
使用干血斑（DBS）检测乙型肝炎病毒（HBV）感染标志物有可能促进 HBV 感染的诊断，尤其是在资源有限的国家。本研究旨在评估 DBS 检测 HBV 感染各种标志物的可行性。
从经过充分特征鉴定的血浆样本中设计了 54 个 DBS 样本。所有 DBS 样本均检测 HBsAg、总抗-HBc 和 HBV DNA，其中 20 个样本还使用市售试剂盒检测 HBeAg。25 个样本中的 24 个（96%）检测到 HBsAg，25 个样本中的 22 个（88%）检测到 HBV DNA，9 个样本中的 9 个（100%）检测到总抗-HBc，7 个样本中的 7 个（100%）检测到 HBeAg。DBS 洗脱液与相应血浆样本之间 HBV DNA 水平的平均差异为 2.7 log IU/mL。15 个 DBS 洗脱液中 HBV DNA 阳性的样本进行了测序，均属于 HBV 基因型 A。13 个未检测到所有 HBV 标志物的样本显示 HBeAg 假阳性。因此，DBS 是检测 HBsAg、总抗-HBc 和 HBV DNA 的可靠样本基质，但不能检测 HBeAg。进一步研究 DBS 用于诊断目的和流行病学研究是必要的。
