Department of Physiology and Oral Physiology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, 734-8553, Japan.
Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University, Fukushima, 960-1295, Japan.
BMC Res Notes. 2022 Sep 5;15(1):285. doi: 10.1186/s13104-022-06175-0.
Omega-6 (n-6) and omega-3 (n-3) polyunsaturated fatty acids (PUFAs) are essential nutrients. Dietary imbalance between these PUFAs, in particular high in n-6 PUFAs and low in n-3 PUFAs (n-6/n-3), is common in modern society. We have previously reported that C57BL/6 mouse male offspring derived from mothers exposed to an n-6/n-3 diet during the gestation had an augmented ventral midbrain dopamine system in adulthood; however, the fatty acid composition in this brain region has not yet been investigated. This follow-up study aims to characterize the fatty acid profile of the ventral midbrain of mice exposed to the n-6/n-3 diet during specific life stages.
n-6 PUFAs, especially linoleic acid, were increased in the ventral midbrain of offspring exposed to the n-6/n-3 diet during the gestation compared to those exposed to a well-balanced control diet throughout life. On the other hand, n-3 PUFAs, especially docosahexaenoic acid, were decreased in the ventral midbrain of offspring exposed to the n-6/n-3 diet during the gestation, lactation, or postweaning period compared to those exposed to the control diet throughout life. Thus, exposure to the n-6/n-3 diet in pregnancy increases linoleic acid and that in any life stage decreases docosahexaenoic acid in the offspring's ventral midbrain.
ω-6（n-6）和 ω-3（n-3）多不饱和脂肪酸（PUFAs）是必需营养素。这些多不饱和脂肪酸之间的饮食失衡，特别是 n-6 PUFAs 含量高而 n-3 PUFAs 含量低（n-6/n-3），在现代社会中很常见。我们之前曾报道过，在孕期暴露于 n-6/n-3 饮食中的 C57BL/6 雄性仔鼠成年后腹侧中脑多巴胺系统增强；然而，该脑区的脂肪酸组成尚未得到研究。本后续研究旨在描述在特定生命阶段暴露于 n-6/n-3 饮食的小鼠腹侧中脑的脂肪酸谱。
与终生暴露于均衡对照饮食的仔鼠相比，在孕期暴露于 n-6/n-3 饮食的仔鼠腹侧中脑中 n-6 PUFAs 增加，尤其是亚油酸。另一方面，在孕期、哺乳期或断奶后暴露于 n-6/n-3 饮食的仔鼠腹侧中脑中 n-3 PUFAs 减少，尤其是二十二碳六烯酸。因此，孕期暴露于 n-6/n-3 饮食会增加亚油酸，而任何生命阶段的暴露都会降低仔鼠腹侧中脑的二十二碳六烯酸。
