Universidade Estadual de Londrina, P.O. Box 6001, Londrina, 86051-990, Brazil.
Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid - Pr 445 Km 380 - Campus Universitário, Cx. Postal 6001, Londrina, Paraná, CEP 86051-980, Brazil.
Curr Microbiol. 2022 Sep 5;79(10):307. doi: 10.1007/s00284-022-03005-1.
Amylases, glycoside hydrolases widely used in several industrial processes, can be produced by many animals, plants, bacteria, and fungi. Fungal amylases from Aspergillus sp. hold remarkable importance in biotechnological applications for presenting a great catalysis efficiency in a wide range of pH and temperature. The production of amylases is mainly dependent on the genetic background of the species, i.e., Aspergillus strains, and abiotic factors. Among the major producers of amylases are the species of Aspergillus section Nigri, including Aspergillus welwitschiae. In this study, Aspergillus welwitschiae strains were evaluated for their ability to produce extracellular amylases. Among the 24 strains, wild Aspergillus welwitschiae UELAs 15.262 and mutant A. welwitschiae UELAs 15.262/35 strains showed greater potential for amylases production. The A. welwitschiae UELAs 15.262 produced more amylases (8645 U/mg) when compared to A. welwitschiae UELAs 15.262/35 (6666 U/mg). The amylases activity from partially purified crude enzymatic extract of A. welwitschiae UELAs 15.262 strain obtained at pH 5.5, 60 °C, resulted in 1.98-fold (3837 U/mg) increase in enzymatic activity. Likewise, the amylases activity from partially purified crude extract of A. welwitschiae UELAs 15.262/35 obtained at pH 5.0, 60 °C resulted in 2.2-fold (9077 U/mg) increase in amylases activity. The presence of metallic ions (Cu and Fe) also provided an increase of amylases activity for both strains. To our knowledge, this is the first study reporting the ability of Aspergillus welwitschiae strains in order to produce amylases.
淀粉是糖苷水解酶的一种，广泛应用于多种工业过程，可由许多动物、植物、细菌和真菌产生。黑曲霉属的真菌淀粉酶在生物技术应用中具有重要意义，因为其在广泛的 pH 和温度范围内具有很高的催化效率。淀粉酶的生产主要依赖于物种的遗传背景，即黑曲霉属菌株，以及非生物因素。主要的淀粉酶生产菌包括黑曲霉节的各种菌，包括威氏曲霉。在这项研究中，评估了威氏曲霉菌株产生胞外淀粉酶的能力。在 24 株菌中，野生威氏曲霉 UELAs 15.262 和突变株 A. welwitschiae UELAs 15.262/35 表现出更大的产酶潜力。与 A. welwitschiae UELAs 15.262/35（6666 U/mg）相比，A. welwitschiae UELAs 15.262 产生的淀粉酶（8645 U/mg）更多。在 pH 5.5、60°C 下获得的威氏曲霉 UELAs 15.262 菌株部分纯化粗酶提取液中的淀粉酶活性，使酶活性提高了 1.98 倍（3837 U/mg）。同样，在 pH 5.0、60°C 下获得的威氏曲霉 UELAs 15.262/35 菌株部分纯化粗酶提取液中的淀粉酶活性使淀粉酶活性提高了 2.2 倍（9077 U/mg）。金属离子（Cu 和 Fe）的存在也为两种菌株的淀粉酶活性提供了提高。据我们所知，这是首次报道威氏曲霉属菌株产生淀粉酶的能力。
