比较和优化使用幼畜血清的牛支原体抗体 ELISA 筛查截断值。

Comparison and optimisation of screening cutoff values for Mycoplasma bovis antibody ELISAs using serum from youngstock.

机构信息

Department of Internal Medicine, Reproduction, and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

出版信息

Vet Rec. 2022 Nov;191(9):e2179. doi: 10.1002/vetr.2179. Epub 2022 Sep 6.

DOI:10.1002/vetr.2179

PMID:36065576

Abstract

BACKGROUND

Mycoplasma bovis-associated disease can cause tremendous production losses, welfare issues and high antimicrobial use. Therefore, screening cattle for M. bovis antibodies before entering the herd is a popular and possibly cost-efficient way to reduce disease introduction. However, interpretation of results can be challenging due to variable accuracy between tests and populations. This study's objectives were to compare the diagnostic test accuracy of three commercially available M. bovis antibody ELISAs (ID-screen, Bio K302 and Bio K432) and to explore optimal cutoff values for screening purposes.

METHODS

A prospective diagnostic test accuracy study was performed on 170 serum samples from youngstock using Bayesian latent class modelling. Samples were categorised using manufacturer and generated cutoff values.

RESULTS

Using the manufacturers' guidelines, ID-screen, Bio K432 and Bio K302 showed 97.6%, 67.4% and 33.6% sensitivity, and 78.8%, 97.6% and 99.1% specificity, respectively. Optimised cutoffs resulted in 94.8%, 82.6% and 78.3% sensitivity, and 94.2%, 92.5% and 79.4% specificity, respectively.

CONCLUSIONS

The highest diagnostic accuracy for detecting M. bovis antibodies was obtained by ID-screen (≥110%). However, by adjusting cutoff values, the sensitivity of Bio-X tests could be markedly increased, making these tests also applicable as screening tools.

LIMITATIONS

Interpretation needs to be careful as antibodies may be linked to both infectious and non-infectious status.

摘要

背景

牛支原体相关疾病可导致巨大的生产损失、福利问题和高抗菌药物使用。因此，在牛群中引入之前对牛进行牛支原体抗体筛查是一种流行且可能具有成本效益的方法，可降低疾病的引入。然而，由于不同测试和群体之间的准确性存在差异，因此对结果的解释可能具有挑战性。本研究的目的是比较三种市售牛支原体抗体 ELISA（ID-screen、Bio K302 和 Bio K432）的诊断测试准确性，并探索用于筛查的最佳截断值。

方法

使用贝叶斯潜在类别建模对来自牛犊的 170 份血清样本进行了前瞻性诊断测试准确性研究。使用制造商和生成的截断值对样本进行分类。

结果

使用制造商的指南，ID-screen、Bio K432 和 Bio K302 的敏感性分别为 97.6%、67.4%和 33.6%，特异性分别为 78.8%、97.6%和 99.1%。优化的截断值分别导致敏感性为 94.8%、82.6%和 78.3%，特异性为 94.2%、92.5%和 79.4%。