Effects of applying nutritional programming at different early stages of Largemouth bass (Micropterus salmoides, Lacepède) development on growth and dietary plant protein utilization.

Plant protein (PP) utilization in fish is limited due to lower digestibility compared to fishmeal (FM) and the presence of antinutritional factors. Its utilization can be improved by nutritional programming (NP), a method wherein a fish is provided a nutritional stimulus early in life which can alter their physiology. NP has been shown to be effective but methods of applying NP are varied and have been applied at different stages of development with different outcomes. To find the optimal timeframe to perform NP in fish early stages Largemouth bass (Micropterus salmoides, Lacepède) were nutritionally programmed at three different ages in early development. In this study bass were programmed with: (1) live food enriched with soybean meal (SBM) from 6 to 15 days post-hatch (dph) (NPL), (2) SBM-based formulated diet from 16 to 25 dph (NPD1) and (3) formulated SBM-diet from 26 to 35 dph (NPD2). After programming, each group was fed FM-diet before being refed SBM-diet from 100 to 172 dph. A positive control (PC) was fed FM-diet throughout. Final average body weight of PC was significantly higher than NPD1 and NPD2 but did not significantly differ from NPL. Overall NPL showed much improved growth and utilization of PP compared to NPD1 and was similar to growth achieved by PC. This study showed an optimum window of time exists wherein NP of Largemouth bass yields the best impact on growth in the larval stage and later in life when fed SBM-diet. Programming should be performed right after mouth opening using enriched live food and can result in growth similar to non-programmed fish fed FM-based diet. Programming effects similar to that of the live food approach can be achieved with formulated diet, however it is crucial that Largemouth bass are of a proper age and sufficiently developed when programmed with dry food or severe impacts on growth can occur.