Department of Environmental & Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.
Methods Mol Biol. 2023;2519:65-72. doi: 10.1007/978-1-0716-2433-3_7.
The comet assay is an effective method for identifying DNA breaks and alkali-labile sites induced by genotoxins. Performed as a single-cell electrophoresis, this assay is especially simplistic, and the results are easily reproducible. DNA breakage can be quantitatively assessed by the induced comet tail regions, which can be measured using a variety of comet software. This protocol will finish within approximately two hours with adequate preparation, and digitized images can be taken using a confocal or standard fluorescence microscopes after staining the cell nucleus with a DNA dye.
彗星实验是一种识别遗传毒物诱导的 DNA 断裂和碱不稳定位点的有效方法。作为单细胞电泳进行时，该实验尤其简单，且结果易于重现。彗星尾部区域的诱导可以定量评估 DNA 断裂，这可以使用各种彗星软件进行测量。本方案在充分准备的情况下，大约在两小时内即可完成，并且在使用 DNA 染料对细胞核进行染色后，可以使用共聚焦或标准荧光显微镜拍摄数字化图像。
