Wang Zenan, Tao Jie, Tian Jun, Wei Jun, Hu Ying
IEEE Trans Biomed Eng. 2023 Mar;70(3):780-788. doi: 10.1109/TBME.2022.3201990. Epub 2023 Feb 17.
Cell orientation is a necessary step in micromanipulation, which significantly affects the outcomes of cell manipulation. Existing cell orientation techniques include the trial-and-error manual approach that suffers from low efficiency, the mechanical contact approaches that have problems of being invasive, and non-contact approaches that are difficult to set up.
This paper proposes a system with a surface-acoustic wave (SAWs) to perform noninvasive cell orientation.
The system employed a SAW chip with a pair of interdigital transducers (IDTs) to rotate embryos around the X and Y axis using acoustic streaming force. Instead of rotating the entire embryos like other methods, the proposed system rotates the cytoplasm alone through the cell chorion.
We evaluated the cellular structure recognition algorithm and the rotation control using 100 embryo images and 30 zebrafish embryos. The system successfully recognized all required cellar structures for visual feedback. Furthermore, it rotated all cells into the desired position, including 26 cases completed within 10s with an orientation angle error of less than 4°. All 30 embryos hatched after manipulation.
The proposed technique can automatically rotate the cytoplasm through the cell chorion noninvasively.
The system provides a starting point for noninvasive cell manipulation tasks, such as fast intracellular structure scanning and analysis, and microinjection.
细胞定向是显微操作中的必要步骤，它会显著影响细胞操作的结果。现有的细胞定向技术包括效率低下的试错手动方法、具有侵入性问题的机械接触方法以及难以设置的非接触方法。
本文提出一种利用表面声波（SAW）进行非侵入性细胞定向的系统。
该系统采用带有一对叉指换能器（IDT）的SAW芯片，利用声流体力使胚胎绕X轴和Y轴旋转。与其他方法不同，该系统不是像其他方法那样旋转整个胚胎，而是仅通过细胞绒毛膜旋转细胞质。
我们使用100张胚胎图像和30条斑马鱼胚胎评估了细胞结构识别算法和旋转控制。该系统成功识别了视觉反馈所需的所有细胞结构。此外，它将所有细胞旋转到所需位置，其中26例在10秒内完成，定向角度误差小于4°。所有30条胚胎在操作后均孵化成功。
所提出的技术可以通过细胞绒毛膜非侵入性地自动旋转细胞质。
该系统为非侵入性细胞操作任务提供了一个起点，例如快速细胞内结构扫描和分析以及显微注射。
