Acoustics Robotics Systems Lab (ARSL), ETH-Zürich, Rüschlikon CH-8803, Switzerland.
Department of Pharmaceutical Sciences, Division of Pharmaceutical Technology, University of Basel, Basel CH-4056, Switzerland.
Sci Adv. 2022 Mar 25;8(12):eabm2785. doi: 10.1126/sciadv.abm2785.
In vivo micromanipulation using ultrasound is an exciting technology with promises for cancer research, brain research, vasculature biology, diseases, and treatment development. In the present work, we demonstrate in vivo manipulation of gas-filled microparticles using zebrafish embryos as a vertebrate model system. Micromanipulation methods often are conducted in vitro, and they do not fully reflect the complex environment associated in vivo. Four piezoelectric actuators were positioned orthogonally to each other around an off-centered fluidic channel that allowed for two-dimensional manipulation of intravenously injected microbubbles. Selective manipulation of microbubbles inside a blood vessel with micrometer precision was achieved without interfering with circulating blood cells. Last, we studied the viability of zebrafish embryos subjected to the acoustic field. This successful high-precision, in vivo acoustic manipulation of intravenously injected microbubbles offers potentially promising therapeutic options.
体内超声微操作是一项令人兴奋的技术，有望应用于癌症研究、大脑研究、脉管生物学、疾病和治疗开发。在本工作中，我们以斑马鱼胚胎为脊椎动物模型系统，展示了体内对充气体微颗粒的操控。微操作方法通常在体外进行，不能完全反映体内相关的复杂环境。四个压电执行器彼此正交定位在偏心流道周围，允许对静脉内注射的微泡进行二维操控。通过微泡在血管内的精确选择性操控，实现了对循环血细胞的无干扰。最后，我们研究了斑马鱼胚胎在声场下的存活情况。这种成功的高精度、体内超声微泡操控为潜在的治疗选择提供了可能。
