College of Chemistry, Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, Jilin University, Qianjin Street 2699, Changchun 130012, China.
Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, Jilin, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jan 5;284:121799. doi: 10.1016/j.saa.2022.121799. Epub 2022 Sep 2.
Mitochondria are the sites of respiration in cells, and they participate in many indispensable biological processes. Because variations in mitochondrial viscosity can lead to dysfunctions of mitochondrial structure and function (and even induce malignant diseases), new sensors that can accurately monitor changes in mitochondrial viscosity are essential. To better investigate these changes, we report the development and evaluation of a novel benzothiophene-quinoline-based fluorescent chemosensor (BQL) that was designed especially for monitoring mitochondrial viscosity. BQL demonstrated a large Stokes shift (minimizing interference from autofluorescence) and a good response to viscosity (using the TICT principle). Moreover, BQL demonstrated little to no pH-dependency, polarity-dependency, or interference from other analytes. Thus, BQL has an excellent specificity for viscosity. BQL was used to monitor viscosity changes in mitochondria induced by ion carriers, and was used to report on viscosity in real time during mitophagy. To sum up, BQL provided a new approach for detecting viscosity in living cells and in vivo. BQL should prove to be an excellent tool for the analysis of viscosity changes in live cells.
线粒体是细胞呼吸的场所，参与许多不可或缺的生物过程。由于线粒体粘度的变化会导致线粒体结构和功能的失调（甚至诱发恶性疾病），因此需要新的传感器来准确监测线粒体粘度的变化。为了更好地研究这些变化，我们报告了一种新型苯并噻吩-喹啉基荧光化学传感器（BQL）的开发和评估，该传感器专门用于监测线粒体粘度。BQL 表现出较大的斯托克斯位移（最大程度地减少了自发荧光的干扰）和对粘度的良好响应（使用 TICT 原理）。此外，BQL 几乎不受 pH 值、极性或其他分析物干扰的影响。因此，BQL 对粘度具有极好的特异性。BQL 用于监测离子载体诱导的线粒体粘度变化，并用于实时报告线粒体自噬过程中的粘度变化。总之，BQL 为检测活细胞和体内的粘度变化提供了一种新方法。BQL 有望成为分析活细胞中粘度变化的优秀工具。
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