The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China.
Department of Chemistry, University of Bath, Bath BA2 7AY, United Kingdom.
ACS Appl Bio Mater. 2021 Mar 15;4(3):2128-2134. doi: 10.1021/acsabm.0c01446. Epub 2021 Jan 14.
Viscosity is a pivotal factor for indicating the dysfunction of the mitochondria. To date, most of the fluorescent probes developed for mitochondrial viscosity have been designed using BODIPY, hemicyanine, or pyridine-based molecular rotors as part of the core structure. Our aim with this research was to extend the range of suitable fluorophores available for the construction of such fluorescent molecular rotors for evaluating the viscosity of mitocondria. Herein, we have developed a green fluorescent protein (GFP)-chromophore-based fluorescent probe () for the detection of mitochondrial viscosity in live cells. exhibited a high sensitivity toward viscosity (from 7.9 cP to 438.4 cP). The "off-on" sensing mechanism of was ascribed to the restricted rotation of single bonds and excited-state C═C double bonds of . Cell studies indicated that targets the mitochondria and that it was able to monitor real-time changes in the viscosity of live HeLa cell mitochondria. Therefore, we propose that can be used as an effective chemosensor for the real-time imaging of mitochondrial viscosity in live cells. Our results clearly demonstrate the utility of such GFP-chromophore-based derivatives for the development of viscosity-sensitive systems.
粘度是指示线粒体功能障碍的关键因素。迄今为止，大多数为线粒体粘度开发的荧光探针都是使用 BODIPY、半花青或吡啶基分子转子作为核心结构的一部分设计的。我们的研究目的是扩展适用于构建此类荧光分子转子以评估线粒体粘度的荧光团的范围。在这里，我们开发了一种基于绿色荧光蛋白（GFP）生色团的荧光探针（），用于检测活细胞中的线粒体粘度。表现出对粘度的高灵敏度（从 7.9 cP 到 438.4 cP）。的“关-开”传感机制归因于单键和生色团的激发态 C═C 双键的受限旋转。细胞研究表明，针对线粒体，并且能够监测活 HeLa 细胞线粒体粘度的实时变化。因此，我们提出可以将其用作实时成像活细胞线粒体粘度的有效化学传感器。我们的结果清楚地证明了基于 GFP-生色团的衍生物在开发粘度敏感系统中的实用性。
