两种基于喹啉的双光子荧光探针，用于活 HeLa 细胞亚细胞器中粘度的成像。
Two quinoline-based two-photon fluorescent probes for imaging of viscosity in subcellular organelles of living HeLa cells.
机构信息
College of Chemistry and Chemical Engineering, Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Anhui University), Ministry of Education, Photoelectric conversion energy materials and devices Key Laboratory of Anhui Province, Anhui University, Hefei 230039, PR China.
College of Chemistry and Chemical Engineering, Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Anhui University), Ministry of Education, Photoelectric conversion energy materials and devices Key Laboratory of Anhui Province, Anhui University, Hefei 230039, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Dec 15;283:121769. doi: 10.1016/j.saa.2022.121769. Epub 2022 Aug 18.
Two viscosity-sensitive two-photon fluorescent probes (QL and QLS) were designed and synthesized, which can be localized in lysosome and mitochondria in living HeLa cells, respectively. As the increases of viscosity from 2.55 to 1150 cP, the fluorescence quantum yield (Φ) of QL and QLS was increased by 28-fold and 37-fold, respectively. At the same time, its effective two-photon absorption cross section (Φδ) was enhanced by 15-fold and 16-fold, respectively. Fluorescence lifetime imaging (FLIM) of living HeLa cells stained with QL and QLS, revealed that lysosomal viscosity ranged from 100.76 to 254.74 cP and mitochondrial viscosity ranged from 92.21 to 286.79 cP. This type of fluorescent probe is helpful in the design and application of materials for monitoring diseases associated with abnormal viscosity.
设计并合成了两种对粘滞度敏感的双光子荧光探针（QL 和 QLS），它们分别能够定位于活 HeLa 细胞的溶酶体和线粒体中。当粘滞度从 2.55 增加到 1150 cP 时，QL 和 QLS 的荧光量子产率（Φ）分别增加了 28 倍和 37 倍。同时，它们的有效双光子吸收截面（Φδ）分别增强了 15 倍和 16 倍。用 QL 和 QLS 染色的活 HeLa 细胞的荧光寿命成像（FLIM）表明，溶酶体的粘滞度范围为 100.76 至 254.74 cP，线粒体的粘滞度范围为 92.21 至 286.79 cP。这种荧光探针有助于设计和应用与异常粘滞度相关的疾病监测材料。
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