Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Int Immunopharmacol. 2022 Nov;112:109204. doi: 10.1016/j.intimp.2022.109204. Epub 2022 Sep 5.
Amomum longiligulare polysaccharide 1 (ALP1) was extracted from Amomum longiligulare T.L. Wu fruits and the poly (lactic-co-glycolic acid) nanoparticle enveloping ALP1 (ALPP) showed a good promoting effect on the activation of macrophages in our previous study. To further understand the immunomodulatory property of ALPP, the effect of ALPP on T lymphocytes and dendritic cells was investigated in the present study. The proliferation rates of chicken T lymphocytes and chicken bone marrow dendritic cells (chBM-DCs) that were treated with ALP1 or ALPP were determined by using MTT method. Meanwhile, the relative mRNA levels of cytokines from T lymphocytes and surface molecules of chBM-DCs were determined by using qRT-PCR method. In addition, the drug uptake capacity of chBM-DCs was also tested. As a result, the promoting effect on the proliferation of T lymphocytes and the Th1-type immune response of ALPP was better than that of ALP1. In addition, ALPP was much more effectively swallowed by chBM-DCs so that its promoting effect on the proliferation and maturation of chBM-DCs was higher than that of ALP1. To conclude, ALPP had a stronger immunomodulatory activity than ALP1, and showed the potential to become a new type of immune booster.
从长角砂仁果实中提取的长角砂仁多糖 1（ALP1），并在我们之前的研究中发现聚（乳酸-共-乙醇酸）纳米粒包裹 ALP1（ALPP）对巨噬细胞的激活有很好的促进作用。为了进一步了解 ALPP 的免疫调节特性，本研究考察了 ALPP 对 T 淋巴细胞和树突状细胞的作用。采用 MTT 法测定 ALP1 或 ALPP 处理的鸡 T 淋巴细胞和鸡骨髓树突状细胞（chBM-DCs）的增殖率。同时，采用 qRT-PCR 法测定 T 淋巴细胞细胞因子和 chBM-DCs 表面分子的相对 mRNA 水平。此外，还检测了 chBM-DCs 的摄取能力。结果表明，ALPP 对 T 淋巴细胞的增殖和 Th1 型免疫应答的促进作用优于 ALP1。此外，ALPP 被 chBM-DCs 更有效地吞噬，因此其对 chBM-DCs 的增殖和成熟的促进作用高于 ALP1。综上所述，ALPP 比 ALP1 具有更强的免疫调节活性，有望成为新型免疫增强剂。
