Chen Yijing, Wu Haowen, Zhang Chenglong, Luo Qiyuan, Chen Yun
Sanya Institute of Breeding and Multiplication, School of Tropical Agriculture and Forestry, Hainan University, Sanya, 572025, P. R. China.
Macromol Biosci. 2025 Jan;25(1):e2400297. doi: 10.1002/mabi.202400297. Epub 2024 Sep 13.
In this study, Amomum longiligulare polysaccharide 1 (ALP1) is used to chelate with magnesium (Mg) to synthesize the ALP1-Mg (II) complex (ALP1-Mg). Based on Box-Behnken response surface design, the optimum technological conditions are 22 mg mL trisodium citrate, 2.10 mol L MgCl, reaction at 70 °C for 2.9 h, resulting in a maximum Mg content of 2.13%. Next, the physicochemical properties and structural characteristics of ALP1 and ALP1-Mg are characterized, and the results show that the morphology, conformation, crystallinity, and thermal stability of ALP1-Mg are changed. In addition, dietary supplementation of 500 mg kg ALP1-Mg significantly reduces the feed conversion ratio during the grower (15-35 d). Meanwhile, the villus height/crypt depth of the duodenum and ileum are significantly increased, and the relative abundance of Lactobacillus is significantly elevated. Taken together, the results suggest that ALP1-Mg is a potential growth-promoting feed additive.
在本研究中，长柄砂仁多糖1（ALP1）与镁（Mg）螯合以合成ALP1 - Mg（II）复合物（ALP1 - Mg）。基于Box - Behnken响应面设计，最佳工艺条件为22 mg/mL柠檬酸钠、2.10 mol/L MgCl，在70°C下反应2.9小时，最大镁含量为2.13%。接下来，对ALP1和ALP1 - Mg的理化性质和结构特征进行了表征，结果表明ALP1 - Mg的形态、构象、结晶度和热稳定性发生了变化。此外，日粮中添加500 mg/kg ALP1 - Mg可显著降低生长育肥期（15 - 35日龄）的饲料转化率。同时，十二指肠和回肠的绒毛高度/隐窝深度显著增加，乳酸杆菌的相对丰度显著提高。综上所述，结果表明ALP1 - Mg是一种潜在的促生长饲料添加剂。
