通过高效液相色谱法对人乳寡糖进行分级分离。
Fractionation of human milk oligosaccharides by high-performance liquid chromatography.
作者信息
Konami Y, Yamamoto K, Tsuji T, Osawa T
出版信息
Biol Chem Hoppe Seyler. 1987 Apr;368(4):309-14. doi: 10.1515/bchm3.1987.368.1.309.
PMID:3606817
Abstract
An ion-exchange chromatographic system was used to isolate several human milk oligosaccharides, the elution being carried out with a linear gradient of a sodium borate buffer. Lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose I, II and III, lacto-N-difucohexaose I and 2'-alpha-fucosyllactose can be separated by this method.
摘要
采用离子交换色谱系统分离几种人乳寡糖，用硼酸钠缓冲液线性梯度进行洗脱。通过该方法可分离出N-四糖乳糖、新乳糖-N-四糖、岩藻糖基-N-五糖I、II和III、二岩藻糖基-N-六糖I和2'-α-岩藻糖基乳糖。
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