Leo Fiame, Asakuma Sadaki, Fukuda Kenji, Senda Akitsugu, Urashima Tadasu
Graduate School of Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
Biosci Biotechnol Biochem. 2010;74(2):298-303. doi: 10.1271/bbb.90614. Epub 2010 Feb 7.
An improved analytical method using reverse-phase high performance liquid chromatography following anthranilic acid derivatization for the measurement of each oligosaccharide level in transition (5 to 10 d lactation) and mature (21 to 155 d lactation) milks of sixteen Samoan women is described. The method was applied for the measurement of sialyl as well as neutral oligosaccharide levels. We found that disialyllacto-N-tetraose (DSLNT), sialylacto-N-tetraose c (LSTc), and 6'-sialyllactose (6'-SL) were the most abundant of the sialyl oligosaccharides. In the neutral oligosaccharide fraction, lacto-N-fucopentaose II and III combined (LNFP II+III) were the most dominant, followed by lacto-N-tetraose (LNT) and 3-fucosyllactose (3-FL). 2'-Fucosyllactose (2'-FL) and lacto-N-fucopentaose I (LNFP I) were absent in some and found at low levels in most of the Samoan women. Our study indicates that the milk oligosaccharide composition in Samoan women is similar to that of Japanese women with respect to sialyl but not to neutral oligosaccharides. The differences in neutral oligosaccharides might have a genetic origin.
描述了一种改进的分析方法，该方法采用邻氨基苯甲酸衍生化后的反相高效液相色谱法，用于测定16名萨摩亚妇女过渡（泌乳5至10天）和成熟（泌乳21至155天）乳汁中各寡糖水平。该方法用于测定唾液酸化寡糖以及中性寡糖水平。我们发现，二唾液酸乳糖-N-四糖（DSLNT）、唾液酸乳糖-N-四糖c（LSTc）和6'-唾液酸乳糖（6'-SL）是最丰富的唾液酸化寡糖。在中性寡糖组分中，乳糖-N-岩藻五糖II和III组合（LNFP II+III）占主导地位，其次是乳糖-N-四糖（LNT）和3-岩藻糖基乳糖（3-FL）。2'-岩藻糖基乳糖（2'-FL）和乳糖-N-岩藻五糖I（LNFP I）在一些萨摩亚妇女中不存在，在大多数萨摩亚妇女中含量较低。我们的研究表明，萨摩亚妇女乳汁中的寡糖组成在唾液酸化方面与日本妇女相似，但在中性寡糖方面则不同。中性寡糖的差异可能源于遗传。
