Asakuma S, Urashima T, Akahori M, Obayashi H, Nakamura T, Kimura K, Watanabe Y, Arai I, Sanai Y
Graduate School of Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
Eur J Clin Nutr. 2008 Apr;62(4):488-94. doi: 10.1038/sj.ejcn.1602738. Epub 2007 Mar 21.
Human colostrum is known to be important for the protection of infants against infection by pathogenic microorganisms. This protection is thought to be due, partially, to various neutral and acidic oligosaccharides that are present in colostrum and milk. However, the concentrations of each of the oligosaccharide of human colostrum have not yet been determined. The aim of this present study was to determine the concentration of each of the major neutral oligosaccharide for three consecutive days from the start of lactation.
We analyzed the level of each neutral oligosaccharide in human colostrum, for three consecutive days from the start of lactation, obtained from 12 healthy Japanese women (ranging in age from 21 to 35 years; primipara 6 and multipara 6). The ABO blood groups of the donors were determined: A, three; B, three; O, five; AB, one. The determined human milk oligosaccharides were 2'-fucosyllactose (2'-FL), 3-fucosyllactose (3-FL), lactodifucotetraose (LDFT), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT), three lacto-N-fucopentaose (LNFP I, II and III) and two lacto-N-difucohexaose (LNFDH I and II) using high-performance liquid chromatography (HPLC) with two derivatization techniques.
The concentrations of 2'-FL and LDFT in colostrum on day 1 were significantly higher than those on days 2 and 3 (P<0.05). An increase in LNT was observed on day 3 compared with day 1 (P<0.05).
These changes in concentrations of 2'-FL, LDFT and LNT may reflect the requirements for prebiotics and anti-infection agents by human infants during early lactation.
众所周知，人初乳对于保护婴儿免受病原微生物感染至关重要。这种保护作用部分被认为归因于初乳和乳汁中存在的各种中性和酸性低聚糖。然而，人初乳中每种低聚糖的浓度尚未确定。本研究的目的是从泌乳开始连续三天测定每种主要中性低聚糖的浓度。
我们分析了12名健康日本女性（年龄在21至35岁之间；初产妇6名，经产妇6名）从泌乳开始连续三天的人初乳中每种中性低聚糖的水平。确定了供体的ABO血型：A型3人；B型3人；O型5人；AB型1人。使用两种衍生技术的高效液相色谱法（HPLC）测定的人乳低聚糖为2'-岩藻糖基乳糖（2'-FL）、3-岩藻糖基乳糖（3-FL）、乳糖二岩藻四糖（LDFT）、乳糖-N-四糖（LNT）、乳糖-N-新四糖（LNnT）、三种乳糖-N-岩藻五糖（LNFP I、II和III）以及两种乳糖-N-二岩藻六糖（LNFDH I和II）。
第1天初乳中2'-FL和LDFT的浓度显著高于第2天和第3天（P<0.05）。与第1天相比，第3天观察到LNT增加（P<0.05）。
2'-FL、LDFT和LNT浓度的这些变化可能反映了人类婴儿在早期泌乳期间对益生元和抗感染剂的需求。
