Chen Ying, Fan Xue-Yun, Jin Yu-Lan, Yao San-Qiao, Yun Xiang, Hua Zheng-Bing, Shen Fu-Hai
School of Public Health, Hebei United University, Tangshan, Hebei, China E-mail :
Asian Pac J Cancer Prev. 2014;15(20):8775-8. doi: 10.7314/apjcp.2014.15.20.8775.
To explore the relationship between polymorphisms of interleukin17 (IL-17) gene(A-832G 7488A/G) and the susceptibility to silicosis, a risk factor for lung cancer.
A total of 113 silicosis patients and 116 workers without silicosis were enrolled in the case-control study. IL-17A A-832G and IL-17F 7488A/G polymorphisms were evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
The frequencies of AA,GG and AG of IL-17A A-832G locus in the case and control groups were 46.9%, 8.0%, 45.1%, and 49.2%, 7.6%, 43.2%, respectively, with no significant differences (p>0.05).The GG genotype in the IL-17F (7488A/G) locus was not found. The frequencies of AA and GA of IL-17F 7488A/G locus in the case and control groups were 84.1%, 15.9% and 66.4%, 33.6%, respectively (p<0.05). Analysis of combined effects showed that the individuals with GG+AG genotype of IL-17A and GG+GA genotype of IL-17F are protected against silicosis (OR=0.469).
IL-17F 7488A/G is associated with susceptibility to silicosis, and G allele may have a protective effect. No relationship was found between IL-17A gene polymorphisms at A-832G and silicosis.
为探讨白细胞介素17（IL-17）基因多态性（A-832G、7488A/G）与矽肺（肺癌的一个危险因素）易感性之间的关系。
病例对照研究共纳入113例矽肺患者和116例无矽肺的工人。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测IL-17A A-832G和IL-17F 7488A/G基因多态性。
病例组和对照组中IL-17A A-832G位点AA、GG和AG基因型频率分别为46.9%、8.0%、45.1%和49.2%、7.6%、43.2%，差异无统计学意义（p>0.05）。未发现IL-17F（7488A/G）位点的GG基因型。病例组和对照组中IL-17F 7488A/G位点AA和GA基因型频率分别为84.1%、15.9%和66.4%、33.6%（p<0.05）。联合效应分析显示，IL-17A的GG+AG基因型和IL-17F的GG+GA基因型个体对矽肺具有保护作用（OR=0.469）。
IL-17F 7488A/G与矽肺易感性相关，G等位基因可能具有保护作用。未发现IL-17A基因A-832G位点多态性与矽肺之间存在关联。
