Mazuran R, Silobrcić V
Ann Inst Pasteur Immunol. 1987 Mar-Apr;138(2):201-11.
As inhibition of spreading of mouse peritoneal macrophages is the basis for an in vitro test of cellular immunity, this test was used to investigate the correlation between in vitro and in vivo results and the kinetics of inhibition of spreading. BALB/c and NIH strain mice were immunized with human or bovine serum albumin or bovine gamma globulin. They displayed a positive footpad test when challenged with the specific antigen (21 days later). Peritoneal cells (PC) of mice with a strong footpad reaction were used for the spreading inhibition test. Intensity of spreading inhibition correlated well (r = -0.93) with that of the footpad reaction. Spreading inhibition had a cyclic pattern. A similar pattern was observed after incubation of PC with preformed lymphokines. The cyclic pattern was probably caused by a repeated action of lymphokines on spread macrophages, resulting in reversible and re-inducible inhibition.
由于小鼠腹腔巨噬细胞扩散的抑制是细胞免疫体外试验的基础，该试验被用于研究体外和体内结果之间的相关性以及扩散抑制的动力学。用人类或牛血清白蛋白或牛γ球蛋白对BALB/c和NIH品系小鼠进行免疫。当用特异性抗原攻击时（21天后），它们表现出阳性足垫试验。具有强烈足垫反应的小鼠的腹腔细胞（PC）用于扩散抑制试验。扩散抑制强度与足垫反应强度具有良好的相关性（r = -0.93）。扩散抑制呈周期性模式。在用预先形成的淋巴因子孵育PC后观察到类似的模式。这种周期性模式可能是由淋巴因子对扩散巨噬细胞的反复作用引起的，导致可逆和可再诱导的抑制。
