Pham Ngoc Minh Quynh, Vuong Quan V, Sakoff Jennette A, Bowyer Michael C, Le Van Anh, Scarlett Christopher J
Department of Applied Sciences, School of Environmental and Life Sciences, University of Newcastle, Brush Rd, Ourimbah, NSW 2258 Australia.
Department of Biotechnology, Institute of Biotechnology and Environment, Nha Trang University, Nha Trang, Vietnam.
3 Biotech. 2022 Oct;12(10):257. doi: 10.1007/s13205-022-03314-z. Epub 2022 Sep 3.
This study aimed to determine the phytochemical, antioxidant, and anticancer activities of the crude extract and its fractions of . The results showed that total phenolic content (TPC), their secondary metabolites (flavonoids-TFC; proanthocyanidins-TPro), and antioxidant activity were significantly different between the crude extract and its fractions. The butanol fraction (F3) had the highest levels of TPC, TFC, and TPro, followed by the crude extract, aqueous fraction (F4), dichloromethyl fraction (F2), and hexane fraction (F1). High-Pressure Liquid Chromatography (HPLC) analysis revealed 14 major bioactive compounds were identified in the extract. Further analysis showed F3 fraction contained the highest levels of the major bioactive compounds, while F1 fraction had the lowest. A similar pattern was observed for antioxidant activities. The crude extract, F3 and F4 fractions were further tested for cytotoxicity against 10 cancer cell lines, including HT29 (colon); U87, SJG2 (glioblastoma); MCF-7 (Breast); A2780 (ovarian); H460 (lung); A431 (skin); Du145 (prostate); BE2-C (neuroblastoma); MIA PaCa-2 (pancreas); and one non-tumour-derived normal breast cell line (MCF10A). Except for Du145 (prostate), the crude extract, F3 and F4 fractions inhibited the cancer cell lines at 100 µg/mL, with F3 possessing greater activity against these cancer cell lines. Future studies are recommended to isolate and identify the major bioactive compounds of the F3 fraction, and further tested their impact against cancer cell lines. This could identify the potential of anticancer agents from .
本研究旨在测定[提取物名称]粗提物及其各馏分的植物化学、抗氧化和抗癌活性。结果表明，粗提物及其各馏分之间的总酚含量（TPC）、其次级代谢产物（黄酮类化合物 - TFC；原花青素 - TPro）和抗氧化活性存在显著差异。正丁醇馏分（F3）的TPC、TFC和TPro含量最高，其次是粗提物、水相馏分（F4）、二氯甲烷馏分（F2）和己烷馏分（F1）。高压液相色谱（HPLC）分析显示，在[提取物名称]提取物中鉴定出14种主要生物活性化合物。进一步分析表明，F3馏分中主要生物活性化合物的含量最高，而F1馏分的含量最低。抗氧化活性也呈现类似模式。粗提物、F3和F4馏分进一步针对10种癌细胞系进行细胞毒性测试，包括HT29（结肠）；U87、SJG2（胶质母细胞瘤）；MCF - 7（乳腺）；A2780（卵巢）；H460（肺）；A431（皮肤）；Du145（前列腺）；BE2 - C（神经母细胞瘤）；MIA PaCa - 2（胰腺）；以及一种非肿瘤来源的正常乳腺细胞系（MCF10A）。除Du145（前列腺）外，粗提物、F3和F4馏分在100μg/mL时对癌细胞系有抑制作用，其中F3对这些癌细胞系具有更强的活性。建议未来的研究分离和鉴定F3馏分中的主要生物活性化合物，并进一步测试它们对癌细胞系的影响。这可能确定从[提取物名称]中提取抗癌剂的潜力。
