Simultaneous determination of phthalates and bisphenols from plastic bottled water samples by dispersive solid-phase extraction with multiwalled carbon nanotubes and liquid chromatography/atmospheric pressure photoionization/high-resolution mass spectrometry.

RATIONALE

Phthalates and bisphenols were reported as endocrine disrupting chemicals and hence a potential threat to human health. Polyethylene terephthalate bottles are being used to store drinking water and the probability of migration of phthalates and bisphenols from the bottles into the water is high. The migration of analytes with respect to different storage conditions need to be studied.

METHOD

A sensitive analytical method for simultaneous determination of seven phthalates and three bisphenols from packaged drinking water was developed using liquid chromatography/atmospheric pressure photoionization/high-resolution mass spectrometry. The analytes were extracted by dispersive solid-phase extraction by multiwalled carbon nanotubes.

RESULTS

The developed method showed linearity from 0.5 to 5000 μg/L with the limit of detection and limit of quantification ranging from 0.5 to 1 μg/L and 1 to 2 μg/L, respectively, for phthalates and bisphenols. The inter- and intraday variations were below 10%. The recoveries were in the range of 79.5% to 112%. The migration of phthalates and bisphenols increased with storage time and temperature. Maximum migration was observed for diisobutyl phthalate of 1209.7 ng/L followed by dibutyl phthalate at 777.8 ng/L on 180 days of analysis at room temperature. Migration of bis(2-ethylhexyl) phthalate was observed to be higher at elevated temperatures, increasing from 14.9 to 514 ng/L. Similarly, migration of bisphenol-A was increased at 45°C. The results were subjected to analysis of variance (ANOVA) studies and the results showed significant variations of phthalates and bisphenols with respect to storage temperature and time.

CONCLUSION

The use of atmospheric pressure photoionization facilitated simultaneous determination of phthalates and bisphenols. The migration of phthalates and bisphenols increased with increasing temperature and storage time. Maximum migration was observed for diethyl, diisobutyl, dibutyl and bis(2-ethylhexyl) phthalates. This may be attributed to the type of plastic, the processing parameters and recycling.