The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Frontiers Science Research Base of Biomimetic Catalysis, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China.
Department of Chemistry, University of Bath, BA2 7AY, UK.
Chem Commun (Camb). 2022 Sep 22;58(76):10727-10730. doi: 10.1039/d2cc00118g.
The occurrence of endoplasmic reticulum (ER) stress is the main cause of a variety of biological processes that are closely related to numerous diseases. The homeostasis of the ER microenvironment can be disrupted under ER stress. In this research, by linking a pentafluorophenyl to the green fluorescent protein chromophore, we have developed a new ER-targeting fluorescent probe (GE-Y) for measuring changes of intracellular ER viscosity caused by ER stress. Importantly, an increase in ER viscosity was observed using GE-Y in cells undergoing autophagy. As such, our research provides an ideal tool for studying ER stress and autophagy.
内质网（ER）应激的发生是与许多疾病密切相关的各种生物学过程的主要原因。ER 微环境的动态平衡可以在 ER 应激下被破坏。在这项研究中，我们通过将五氟苯基连接到绿色荧光蛋白生色团上，开发了一种新的 ER 靶向荧光探针（GE-Y），用于测量 ER 应激引起的细胞内 ER 粘度变化。重要的是，在用 GE-Y 处理发生自噬的细胞时，观察到 ER 粘度增加。因此，我们的研究为研究 ER 应激和自噬提供了理想的工具。
