Sichuan University Library, Sichuan University, 610041, Chengdu, P. R. China.
Department of Laboratory Medicine, State Key Laboratory of Biotherapy and Cancer Center, Med-X Center for Manufacturing, West China Hospital, Sichuan University, 610041, Chengdu, P. R. China.
Chem Asian J. 2022 Nov 16;17(22):e202200774. doi: 10.1002/asia.202200774. Epub 2022 Sep 26.
Pathogen infections seriously threaten human health, and there is an urgent demand for rapid and efficient pathogen identification to provide instructions in clinical diagnosis and therapeutic intervention. Recently, nanopore technology, a rapidly maturing technology which delivers ultrasensitive sensing and high throughput in real-time and at low cost, has achieved success in pathogen detection. Furthermore, the remarkable development of nanopore sequencing, for example, the MinION sequencer from Oxford Nanopore Technologies (ONT) as a competitive sequencing technology, has facilitated the rapid analysis of disease-related microbiomes at the whole-genome level and on a large scale. Here, we highlighted recent advances in nanopore approaches for pathogen detection at the single-molecule level. We also overviewed the applications of nanopore sequencing in pathogenic bacteria identification and diagnosis. In the end, we discussed the challenges and future developments of nanopore technology as promising tools for the management of infections, which may be helpful to aid understanding as well as decision-making.
病原体感染严重威胁人类健康，因此迫切需要快速、高效的病原体鉴定方法，为临床诊断和治疗干预提供指导。近年来，纳米孔技术作为一种快速发展的技术，在实时、低成本的情况下实现了超灵敏传感和高通量，在病原体检测方面取得了成功。此外，纳米孔测序技术取得了显著进展，例如牛津纳米孔技术公司（ONT）的 MinION 测序仪作为一种具有竞争力的测序技术，促进了疾病相关微生物组在全基因组水平和大规模上的快速分析。在这里，我们重点介绍了纳米孔技术在单分子水平上进行病原体检测的最新进展。我们还概述了纳米孔测序在病原菌鉴定和诊断中的应用。最后，我们讨论了纳米孔技术作为感染管理有前途的工具所面临的挑战和未来发展，这可能有助于辅助理解和决策。
